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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab129090 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- SQSTM1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human SQSTM1 Antibody,ÊMonoclonal (C1),Êcould be used forÊWB,ÊIF, ICC and so on.ApplicationáÊWB:Ê 0.2-2µg/mLáÊIF/ICC:Ê 2-20µg/mLProtein FunctionAdapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Val136~Gln400- Antibody clone number
- C1
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- SQSTM1 Mouse mAb (Ab129090) - Western Blot All lanes: SQSTM1 Mouse mAb (Ab129090) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 54 kDa Exposure time: 1.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- SQSTM1 Mouse mAb (Ab129090) - IF Immunocytochemistry analysis of SQSTM1/p62 (green) in U87-MG cells using SQSTM1 Mouse mAb (Ab129090), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- SQSTM1 Mouse mAb (Ab129090) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SQSTM1 Mouse mAb (Ab129090). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
