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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10523 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10523, RRID:AB_11188768
- Product name
- CCT2 monoclonal antibody, clone 5B5C4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant CCT2.
- Isotype
- IgG
- Antibody clone number
- 5B5C4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against HeLa (1), MCF-7 (2), Jurkat (3), T-47D (4), K-562 (5), A-431 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against recombinant protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (blue) and negative control (red).
- Validation comment
- Flow Cytometry
