R32477

antibody from NSJ Bioreagents

Targeting: CCT2 Cctb

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32477

Provider

NSJ Bioreagents

Product name

TCP1 beta Antibody / CCT2

Antibody type

Polyclonal

Description

This highly specific TCP1 beta antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

Prior to reconstitution, store at 4oC. After reconstitution, the TCP1 beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis, 2) mouse spleen and 3) human SW620 lysate with TCP1 beta antibody. Predicted/observed molecular weight ~57 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain, 2) rat spleen, 3) rat C6, 4) rat PC-12, 5) mouse brain, 6) mouse spleen, 7) mouse NIH 3T3 and 8) mouse RAW264.7 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) A549, 2) RT4, 3) Caco-2, 4) 293T, 5) MCF7, 6) HEL, 7) Raji and 8) SW620 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with TCP1 beta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human testis tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse stomach with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat stomach with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat gastric tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse gastric tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-251 cells with TCP1 beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 beta antibody.