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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06684SU-N - Provider product page
- Provider
- OriGene
- Product name
- TCP1 beta (CCT2) mouse monoclonal antibody, clone 5B5C4, Ascites
- Antibody type
- Monoclonal
- Description
- TCP1 beta (CCT2) mouse monoclonal antibody, clone 5B5C4, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CCT2
- Isotype
- IgG
- Antibody clone number
- 5B5C4
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using CCT2 mAb against Human CCT2 (AA: 87-290) recombinant protein. (Expected MW is 47.9 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using CCT2 Mouse mAb against Hela (Lane 1), MCF-7 (Lane 2), Jurkat (Lane 3), T47D (Lane 4), K562 (Lane 5), A431 (Lane 6), NIH/3T3 (Lane 7), PC-12 (Lane 8) and Cos7 (Lane 9) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using CCT2 Mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using CCT2 Mouse mAb (blue) and Negative Control (red).
- Validation comment
- FC
