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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-93047 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TCP-1 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GGSLADSYLD EGFLLDKKIG VNQPKRIENA KILIANTGMD TDKIKIFGSR VRVDSTAKVA EIEHAEKEKM KEKVERILKH GINCFINRQL IYNYPEQLFG AAGVMAIEHA DFAGVERLAL VTGGEIASTF DHPELVKLGS CKLIEEVMIG EDKLIHFSGV ALGEACTIVL RGATQQILDE AERSLHDALC VLAQTVKDSR TVYGGGCSEM LMAHAVTQLA NRTPGKEAVA MESYAKALRM LPTIIADNAG YDSADLVAQL RAAHSEGNTT AGLDMREGTI GDMAILGITE SFQVKRQVLL SAAEAAEVIL RVDNIIKAAP RKRVPDHHPC; Positive Samples: MCF7, SKOV3, A-549, HT-29, Mouse spleen, Mouse ovary, Mouse heart; Cellular Location: Cytoplasm
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.26 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TCP-1 beta in extracts of various cell lines using TCP-1 beta Polyclonal Antibody (Product # PA5-93047) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TCP-1 beta was performed in HeLa cells using TCP-1 beta Polyclonal Antibody (Product # PA5-93047).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of TCP-1 beta was performed in 200 µg extracts of A-549 cells using TCP-1 beta Polyclonal Antibody (Product # PA5-93047). Western blot was performed from the immunoprecipitate using TCP-1 beta Polyclonal Antibody.
