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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-22221 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TCP-1 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (93%), Pig (100%), Rabbit (100%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TCP-1 beta using 30 µg of A) HepG2 and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TCP-1 beta polyclonal antibody (Product # PA5-22221) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TCP-1 beta Polyclonal Antibody (Product # PA5-22221). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. TCP-1 beta Polyclonal Antibody (Product # PA5-22221) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TCP-1 beta Polyclonal Antibody (Product # PA5-22221). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP-1 beta Polyclonal Antibody (Product # PA5-22221) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TCP-1 beta Polyclonal Antibody (Product # PA5-22221). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP-1 beta Polyclonal Antibody (Product # PA5-22221) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TCP1 beta (Product # PA5-22221) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
