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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [17]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- 101-M40 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Podoplanin
- Antibody type
- Monoclonal
- Antigen
- gp36 (Podoplanin)-expressing MDCK cells
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- (#18H5)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer.
Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type
DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)
Podoplanin-Positive Fibroblasts Enhance Lung Adenocarcinoma Tumor Formation: Podoplanin in Fibroblast Functions for Tumor Progression
Aberrant lymphatic development in euploid fetuses with increased nuchal translucency including Noonan syndrome
Lack of Evidence for the Direct Activation of Endothelial Cells by Adult Female and Microfilarial Excretory-Secretory Products
Heterogeneous expression of toll-like receptors in lymphatic endothelial cells derived from different tissues
Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2
Endothelin-1 Stimulates Lymphatic Endothelial Cells and Lymphatic Vessels to Grow and Invade
Adventitial lymphatics of internal carotid artery in healthy and atherosclerotic vessels.
U94 of human herpesvirus 6 inhibits in vitro angiogenesis and lymphangiogenesis
Podoplanin, a novel marker of tumor-initiating cells in human squamous cell carcinoma A431
Characterization of Anti-podoplanin Monoclonal Antibodies: Critical Epitopes for Neutralizing the Interaction Between Podoplanin and CLEC-2
Lymphatic vessels identified with podoplanin. Comparison of immunostaining with three different detection systems.
Similarities and differences of human and experimental mouse lymphangiomas
Melanoma contains CD133 and ABCG2 positive cells with enhanced tumourigenic potential
Investigation of intratumoural and peritumoural lymphatics expressed by podoplanin and LYVE-1 in the hybridoma-induced tumours
Tokumoto MW, Tanaka H, Tauchi Y, Kasashima H, Kurata K, Yashiro M, Sakurai K, Toyokawa T, Kubo N, Amano R, Kimura K, Muguruma K, Maeda K, Ohira M, Hirakawa K
British journal of cancer 2015 Sep 29;113(7):1046-54
British journal of cancer 2015 Sep 29;113(7):1046-54
Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type
Steiniger B, Wilhelmi V, Seiler A, Lampp K, Stachniss V
Immunology 2014 November;143(3):462-477
Immunology 2014 November;143(3):462-477
DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)
Quentmeier H, Eberth S, Romani J, Weich H, Zaborski M, Drexler H
BMC Cancer 2012 ;12(1):19
BMC Cancer 2012 ;12(1):19
Podoplanin-Positive Fibroblasts Enhance Lung Adenocarcinoma Tumor Formation: Podoplanin in Fibroblast Functions for Tumor Progression
Hoshino A, Ishii G, Ito T, Aoyagi K, Ohtaki Y, Nagai K, Sasaki H, Ochiai A
Cancer Research 2011 July;71(14):4769-4779
Cancer Research 2011 July;71(14):4769-4779
Aberrant lymphatic development in euploid fetuses with increased nuchal translucency including Noonan syndrome
de Mooij Y, van den Akker N, Bekker M, Bartelings M, van Vugt J, Gittenberger-de Groot A
Prenatal Diagnosis 2011 February;31(2):159-166
Prenatal Diagnosis 2011 February;31(2):159-166
Lack of Evidence for the Direct Activation of Endothelial Cells by Adult Female and Microfilarial Excretory-Secretory Products
Weinkopff T, Lammie P, Diemert D
PLoS ONE 2011 August;6(8)
PLoS ONE 2011 August;6(8)
Heterogeneous expression of toll-like receptors in lymphatic endothelial cells derived from different tissues
Garrafa E, Imberti L, Tiberio G, Prandini A, Giulini S, Caimi L
Immunology and Cell Biology 2010 October;89(3):475-481
Immunology and Cell Biology 2010 October;89(3):475-481
Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2
Chaipan C, Steffen I, Tsegaye T, Bertram S, Glowacka I, Kato Y, Schmökel J, Münch J, Simmons G, Gerardy-Schahn R, Pöhlmann S
Retrovirology 2010 ;7(1):47
Retrovirology 2010 ;7(1):47
Endothelin-1 Stimulates Lymphatic Endothelial Cells and Lymphatic Vessels to Grow and Invade
Spinella F, Garrafa E, Di Castro V, Rosano L, Nicotra M, Caruso A, Natali P, Bagnato A
Cancer Research 2009 March;69(6):2669-2676
Cancer Research 2009 March;69(6):2669-2676
Adventitial lymphatics of internal carotid artery in healthy and atherosclerotic vessels.
Drozdz K, Janczak D, Dziegiel P, Podhorska M, Patrzałek D, Ziółkowski P, Andrzejak R, Szuba A
Folia Histochemica et Cytobiologica 2009 January;46(4)
Folia Histochemica et Cytobiologica 2009 January;46(4)
U94 of human herpesvirus 6 inhibits in vitro angiogenesis and lymphangiogenesis
Caruso A, Caselli E, Fiorentini S, Rotola A, Prandini A, Garrafa E, Saba E, Alessandri G, Cassai E, Di Luca D
Proceedings of the National Academy of Sciences 2009 December;106(48):20446-20451
Proceedings of the National Academy of Sciences 2009 December;106(48):20446-20451
Podoplanin, a novel marker of tumor-initiating cells in human squamous cell carcinoma A431
Atsumi N, Ishii G, Kojima M, Sanada M, Fujii S, Ochiai A
Biochemical and Biophysical Research Communications 2008 August;373(1):36-41
Biochemical and Biophysical Research Communications 2008 August;373(1):36-41
Characterization of Anti-podoplanin Monoclonal Antibodies: Critical Epitopes for Neutralizing the Interaction Between Podoplanin and CLEC-2
Ogasawara S, Kaneko M, Price J, Kato Y
Hybridoma 2008 August;27(4):259-267
Hybridoma 2008 August;27(4):259-267
Lymphatic vessels identified with podoplanin. Comparison of immunostaining with three different detection systems.
Cîmpean AM, Raica M, Izvernariu DA, Tătucu D
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2007;48(2):139-43
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2007;48(2):139-43
Similarities and differences of human and experimental mouse lymphangiomas
Kasten P, Schnöink G, Bergmann A, Papoutsi M, Buttler K, Rössler J, Weich H, Wilting J
Developmental Dynamics 2007 October;236(10):2952-2961
Developmental Dynamics 2007 October;236(10):2952-2961
Melanoma contains CD133 and ABCG2 positive cells with enhanced tumourigenic potential
Monzani E, Facchetti F, Galmozzi E, Corsini E, Benetti A, Cavazzin C, Gritti A, Piccinini A, Porro D, Santinami M, Invernici G, Parati E, Alessandri G, La Porta C
European Journal of Cancer 2007 March;43(5):935-946
European Journal of Cancer 2007 March;43(5):935-946
Investigation of intratumoural and peritumoural lymphatics expressed by podoplanin and LYVE-1 in the hybridoma-induced tumours
Ji R, Eshita Y, Kato S
International Journal of Experimental Pathology 2007 August;88(4):257-270
International Journal of Experimental Pathology 2007 August;88(4):257-270
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the momoclonal (#101-M40) antibodies. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.
- Sample type
- Cell lysate
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis with total lysate of different LEC cell lines and the neuroblastoma cell line Sy5y using a monoclonal mouse anti-human Podoplanin antibody [Cat# 101-M40]. The LEC cell line LyLec12 and the neuroblastoma cell line Sy5y showed no signal for Podoplanin expression. The SDS-PAGE was performed under non-reducing conditions.
- Sample type
- cell lysate
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis with total lysate of different LEC cell lines using a monoclonal mouse anti-human Podoplanin antibody [Cat# 101-M40]. The proteins have been denaturated at 95°C for 5 min. The LEC cell lines HDLEC-c5 and LyLec12 showed no signal for Podoplanin expression.
- Sample type
- cell lysate
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Podoplanin (0,5µg/ml) [Cat# 101-M40] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:400.
- Sample type
- HDLEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-mouse IgG-PE was used.
- Sample type
- HDLEC
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with HDMVEC and HUVEC.
- Sample type
- HDMVEC and HUVEC
