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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- 101-M41 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Podoplanin
- Antibody type
- Monoclonal
- Antigen
- membrane protein fraction of isolated human glomeruli
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- (#4D5aE5E6)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Robust Acquisition of Spatial Transcriptional Programs in Tissues With Immunofluorescence-Guided Laser Capture Microdissection.
Association Between c-Met and Lymphangiogenic Factors in Patients With Colorectal Cancer.
Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type
Zhang X, Hu C, Huang C, Wei Y, Li X, Hu M, Li H, Wu J, Czajkowsky DM, Guo Y, Shao Z
Frontiers in cell and developmental biology 2022;10:853188
Frontiers in cell and developmental biology 2022;10:853188
Association Between c-Met and Lymphangiogenic Factors in Patients With Colorectal Cancer.
Kim HJ, Baek MJ, Kang DH, Lee SC, Bae SB, Lee KT, Lee N, Kim H, Jeong D, Ahn TS, Lee MS, Hong DS, Won JH
Annals of coloproctology 2018 Apr;34(2):88-93
Annals of coloproctology 2018 Apr;34(2):88-93
Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type
Steiniger B, Wilhelmi V, Seiler A, Lampp K, Stachniss V
Immunology 2014 November;143(3):462-477
Immunology 2014 November;143(3):462-477
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western blot analysis with recombinant human, mouse and rat soluble Podoplanin. There is a strong cross reaction with rat Podoplanin but not with the mouse form.
- Sample type
- Purified recombinant proteins
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis with total lysate of different LEC cell lines and the neuroblastoma cell line CHP100 using a monoclonal mouse anti-human Podoplanin antibody [Cat# 101-M41]. All LEC cell lines are positive for Podoplanin whereas the neuroblastoma cell CHP100 is negative for Podoplanin expression. The WB was performed under non reducing conditions.
- Sample type
- cell lysates
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis with total lysate of different LEC cell lines using a monoclonal mouse anti-human Podoplanin antibody [Cat# 101-M41]. The proteins have been denaturated at 95°C for 5 min.
- Sample type
- cell lysate
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Podoplanin (1µg/ml) [Cat# 101-M41] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:400.
- Sample type
- HDLEC
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of lymphatic endothelial cells in normal human kidney tissue with anti-human Podoplanin [Cat# 101-M41].
- Sample type
- Human kidney tissue
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Staining of lymphatic endothelial cells in normal human kidney tissue (paraffin-embedded tissue-sections fixed in formalin) with anti-human Podoplanin [Cat# 101-M41].
- Sample type
- Human kidney tissue
