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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [30]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF3244 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Podoplanin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth.
An important role of podoplanin in hair follicle growth.
Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis.
Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis.
Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice.
Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis.
Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival.
Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response.
Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19.
Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression.
Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing.
Salient type 1 interleukin 1 receptor expression in peripheral non-immune cells.
Unexpected contribution of lymphatic vessels to promotion of distant metastatic tumor spread.
Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice.
Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1.
Postnatal Deletion of Podoplanin in Lymphatic Endothelium Results in Blood Filling of the Lymphatic System and Impairs Dendritic Cell Migration to Lymph Nodes.
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD.
Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro.
CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
Type I Interferons Direct Gammaherpesvirus Host Colonization.
Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis.
Effects of adenosine on lymphangiogenesis.
Aging exacerbates damage and delays repair of alveolar epithelia following influenza viral pneumonia.
Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress.
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Regeneration of alveolar type I and II cells from Scgb1a1-expressing cells following severe pulmonary damage induced by bleomycin and influenza.
Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage.
The appearance and modulation of osteocyte marker expression during calcification of vascular smooth muscle cells.
Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation.
Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.
Yoon SY, Dieterich LC, Karaman S, Proulx ST, Bachmann SB, Sciaroni C, Detmar M
PloS one 2019;14(7):e0220341
PloS one 2019;14(7):e0220341
An important role of podoplanin in hair follicle growth.
Yoon SY, Dieterich LC, Tacconi C, Sesartic M, He Y, Brunner L, Kwon O, Detmar M
PloS one 2019;14(7):e0219938
PloS one 2019;14(7):e0219938
Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis.
Tanaka H, Horioka K, Yamamoto M, Asari M, Okuda K, Yamazaki K, Shimizu K, Ogawa K
Cancer science 2019 Sep;110(9):2748-2759
Cancer science 2019 Sep;110(9):2748-2759
Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis.
Chen P, Wang S, Janardhan KS, Zemans RL, Deng W, Karmaus P, Shen S, Sunday M, Que LG, Fessler MB, Zhong XP
Journal of immunology (Baltimore, Md. : 1950) 2019 Sep 1;203(5):1208-1217
Journal of immunology (Baltimore, Md. : 1950) 2019 Sep 1;203(5):1208-1217
Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice.
Li X, Bean A, Uehara M, Banouni N, Ben Nasr M, Kasinath V, Jiang L, Fiorina P, Abdi R
Scientific reports 2019 Jul 5;9(1):9778
Scientific reports 2019 Jul 5;9(1):9778
Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis.
Staines KA, Ikpegbu E, Törnqvist AE, Dillon S, Javaheri B, Amin AK, Clements DN, Buttle DJ, Pitsillides AA, Farquharson C
BMC musculoskeletal disorders 2019 Jul 27;20(1):344
BMC musculoskeletal disorders 2019 Jul 27;20(1):344
Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival.
Uehara M, Li X, Sheikhi A, Zandi N, Walker B, Saleh B, Banouni N, Jiang L, Ordikhani F, Dai L, Yonar M, Vohra I, Kasinath V, Orgill DP, Khademhosseini A, Annabi N, Abdi R
Scientific reports 2019 Apr 25;9(1):6535
Scientific reports 2019 Apr 25;9(1):6535
Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response.
Westendorp BF, Büller NVJA, Karpus ON, van Dop WA, Koster J, Versteeg R, Koelink PJ, Snel CY, Meisner S, Roelofs JJTH, Uhmann A, Ver Loren van Themaat E, Heijmans J, Hahn H, Muncan V, Wildenberg ME, van den Brink GR
Cellular and molecular gastroenterology and hepatology 2018;5(1):67-82.e1
Cellular and molecular gastroenterology and hepatology 2018;5(1):67-82.e1
Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19.
Liu L, Zhao L, Yang Y, Gao J, Hu C, Guo B, Zhu B
Oncology letters 2018 Oct;16(4):5013-5019
Oncology letters 2018 Oct;16(4):5013-5019
Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression.
Harris AR, Perez MJ, Munson JM
BMC cancer 2018 Jul 6;18(1):718
BMC cancer 2018 Jul 6;18(1):718
Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing.
Maarouf OH, Uehara M, Kasinath V, Solhjou Z, Banouni N, Bahmani B, Jiang L, Yilmam OA, Guleria I, Lovitch SB, Grogan JL, Fiorina P, Sage PT, Bromberg JS, McGrath MM, Abdi R
JCI insight 2018 Jul 12;3(13)
JCI insight 2018 Jul 12;3(13)
Salient type 1 interleukin 1 receptor expression in peripheral non-immune cells.
Song A, Zhu L, Gorantla G, Berdysz O, Amici SA, Guerau-de-Arellano M, Madalena KM, Lerch JK, Liu X, Quan N
Scientific reports 2018 Jan 15;8(1):723
Scientific reports 2018 Jan 15;8(1):723
Unexpected contribution of lymphatic vessels to promotion of distant metastatic tumor spread.
Ma Q, Dieterich LC, Ikenberg K, Bachmann SB, Mangana J, Proulx ST, Amann VC, Levesque MP, Dummer R, Baluk P, McDonald DM, Detmar M
Science advances 2018 Aug;4(8):eaat4758
Science advances 2018 Aug;4(8):eaat4758
Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice.
Henderson MW, Madenspacher JH, Whitehead GS, Thomas SY, Aloor JJ, Gowdy KM, Fessler MB
Environmental health perspectives 2017 Sep 28;125(9):097024
Environmental health perspectives 2017 Sep 28;125(9):097024
Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1.
Johnson LA, Banerji S, Lawrance W, Gileadi U, Prota G, Holder KA, Roshorm YM, Hanke T, Cerundolo V, Gale NW, Jackson DG
Nature immunology 2017 Jul;18(7):762-770
Nature immunology 2017 Jul;18(7):762-770
Postnatal Deletion of Podoplanin in Lymphatic Endothelium Results in Blood Filling of the Lymphatic System and Impairs Dendritic Cell Migration to Lymph Nodes.
Bianchi R, Russo E, Bachmann SB, Proulx ST, Sesartic M, Smaadahl N, Watson SP, Buckley CD, Halin C, Detmar M
Arteriosclerosis, thrombosis, and vascular biology 2017 Jan;37(1):108-117
Arteriosclerosis, thrombosis, and vascular biology 2017 Jan;37(1):108-117
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD.
Baarsma HA, Skronska-Wasek W, Mutze K, Ciolek F, Wagner DE, John-Schuster G, Heinzelmann K, Günther A, Bracke KR, Dagouassat M, Boczkowski J, Brusselle GG, Smits R, Eickelberg O, Yildirim AÖ, Königshoff M
The Journal of experimental medicine 2017 Jan;214(1):143-163
The Journal of experimental medicine 2017 Jan;214(1):143-163
Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro.
Zheng D, Soh BS, Yin L, Hu G, Chen Q, Choi H, Han J, Chow VT, Chen J
Scientific reports 2017 Jan 27;7:41661
Scientific reports 2017 Jan 27;7:41661
CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
Tan CSE, Lawler C, Stevenson PG
PLoS pathogens 2017 Apr;13(4):e1006311
PLoS pathogens 2017 Apr;13(4):e1006311
Type I Interferons Direct Gammaherpesvirus Host Colonization.
Tan CS, Lawler C, May JS, Belz GT, Stevenson PG
PLoS pathogens 2016 May;12(5):e1005654
PLoS pathogens 2016 May;12(5):e1005654
Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis.
Hintermann E, Bayer M, Ehser J, Aurrand-Lions M, Pfeilschifter JM, Imhof BA, Christen U
Cell adhesion & migration 2016 Jul 3;10(4):419-33
Cell adhesion & migration 2016 Jul 3;10(4):419-33
Effects of adenosine on lymphangiogenesis.
Lenoir B, Wagner DR, Blacher S, Sala-Newby GB, Newby AC, Noel A, Devaux Y
PloS one 2014;9(3):e92715
PloS one 2014;9(3):e92715
Aging exacerbates damage and delays repair of alveolar epithelia following influenza viral pneumonia.
Yin L, Zheng D, Limmon GV, Leung NH, Xu S, Rajapakse JC, Yu H, Chow VT, Chen J
Respiratory research 2014 Sep 30;15(1):116
Respiratory research 2014 Sep 30;15(1):116
Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress.
Sturrock A, Mir-Kasimov M, Baker J, Rowley J, Paine R 3rd
The Journal of biological chemistry 2014 Feb 14;289(7):4095-105
The Journal of biological chemistry 2014 Feb 14;289(7):4095-105
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, Ferron SR, Herault Y, Pavlovic G, Ferguson-Smith AC, Watt FM
Nature 2013 Dec 12;504(7479):277-281
Nature 2013 Dec 12;504(7479):277-281
Regeneration of alveolar type I and II cells from Scgb1a1-expressing cells following severe pulmonary damage induced by bleomycin and influenza.
Zheng D, Limmon GV, Yin L, Leung NH, Yu H, Chow VT, Chen J
PloS one 2012;7(10):e48451
PloS one 2012;7(10):e48451
Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage.
Thomas SN, Rutkowski JM, Pasquier M, Kuan EL, Alitalo K, Randolph GJ, Swartz MA
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2181-90
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2181-90
The appearance and modulation of osteocyte marker expression during calcification of vascular smooth muscle cells.
Zhu D, Mackenzie NC, Millán JL, Farquharson C, MacRae VE
PloS one 2011;6(5):e19595
PloS one 2011;6(5):e19595
Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation.
Peters A, Pitcher LA, Sullivan JM, Mitsdoerffer M, Acton SE, Franz B, Wucherpfennig K, Turley S, Carroll MC, Sobel RA, Bettelli E, Kuchroo VK
Immunity 2011 Dec 23;35(6):986-96
Immunity 2011 Dec 23;35(6):986-96
Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.
Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivée B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A
The Journal of cell biology 2010 Jan 11;188(1):115-30
The Journal of cell biology 2010 Jan 11;188(1):115-30
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Podoplanin by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Podoplanin in Mouse Kidney. Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
