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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AM03203PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM03203PU-S, RRID:AB_1621313
- Product name
- anti Podoplanin (1-206)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human Podoplanin/PDPN (1-206 aa) purified from E. coli
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5E2
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Extracts of mouse kidney (50 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human Podoplanin antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM03203PU-N Podoplanin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cerebellum at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM03203PU-N Podoplanin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
