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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Functional assay [1]
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Validation data
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- Product number
- 103-M40 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Podoplanin
- Antibody type
- Monoclonal
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Mouse
- Host
- Hamster
- Isotype
- IgG
- Antibody clone number
- (#RTD4E10)
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Inflammation and Lymphedema Are Exacerbated and Prolonged by Neuropilin 2 Deficiency
Phenotypic transformation of intimal and adventitial lymphatics in atherosclerosis: a regulatory role for soluble VEGF receptor 2
Neuropilin 1 Receptor Is Up-Regulated in Dysplastic Epithelium and Oral Squamous Cell Carcinoma
Identification of a New Stromal Cell Type Involved in the Regulation of Inflamed B Cell Follicles
Unusual timing of CD127 expression by mouse uterine natural killer cells
Does Plasminogen Activator Inhibitor-1 Drive Lymphangiogenesis?
Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels
Mucka P, Levonyak N, Geretti E, Zwaans B, Li X, Adini I, Klagsbrun M, Adam R, Bielenberg D
The American Journal of Pathology 2016 November;186(11):2803-2812
The American Journal of Pathology 2016 November;186(11):2803-2812
Phenotypic transformation of intimal and adventitial lymphatics in atherosclerosis: a regulatory role for soluble VEGF receptor 2
Taher M, Nakao S, Zandi S, Melhorn M, Hayes K, Hafezi-Moghadam A
The FASEB Journal 2016 July;30(7):2490-2499
The FASEB Journal 2016 July;30(7):2490-2499
Neuropilin 1 Receptor Is Up-Regulated in Dysplastic Epithelium and Oral Squamous Cell Carcinoma
Shahrabi-Farahani S, Gallottini M, Martins F, Li E, Mudge D, Nakayama H, Hida K, Panigrahy D, D'Amore P, Bielenberg D
The American Journal of Pathology 2016 April;186(4):1055-1064
The American Journal of Pathology 2016 April;186(4):1055-1064
Identification of a New Stromal Cell Type Involved in the Regulation of Inflamed B Cell Follicles
Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli M, Ruddle N, Nowak J, Aurrand-Lions M, Luche H, Bajénoff M, Bhandoola A
PLoS Biology 2013 October;11(10)
PLoS Biology 2013 October;11(10)
Unusual timing of CD127 expression by mouse uterine natural killer cells
Zhang J, Chen Z, Fritz J, Rochman Y, Leonard W, Gommerman J, Plumb A, Abraham N, Croy B
Journal of Leukocyte Biology 2012 March;91(3):417-426
Journal of Leukocyte Biology 2012 March;91(3):417-426
Does Plasminogen Activator Inhibitor-1 Drive Lymphangiogenesis?
Bruyère F, Melen-Lamalle L, Blacher S, Detry B, Masset A, Lecomte J, Lambert V, Maillard C, Høyer-Hansen G, Lund L, Foidart J, Noël A, Cao Y
PLoS ONE 2010 March;5(3)
PLoS ONE 2010 March;5(3)
Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels
Schniedermann J, Rennecke M, Buttler K, Richter G, Städtler A, Norgall S, Badar M, Barleon B, May T, Wilting J, Weich H
BMC Cell Biology 2010 ;11(1):50
BMC Cell Biology 2010 ;11(1):50
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human, mouse and rat Podoplanin using a monoclonal anti-mouse Podoplanin antibody [Cat# 103-M40]. There is no cross reactivity with human and rat Podoplanin.
- Sample type
- purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary mouse lung microvascular endothelial cells (MLMEC)
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Functional ELISA with anti-mouse Podoplanin [Cat# 103-M40]. Recombinant mouse soluble Podoplanin was coated with increasing amounts in a 96 well microtiter plate.
