PA5-82301

antibody from Invitrogen Antibodies

Targeting: PDPN aggrus, Gp38, GP40, PA2.26, T1A-2

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-82301

Provider

Invitrogen Antibodies

Product name

Podoplanin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RLPRVWEARA PSLSGAPAPT PPAPPPSRSS RLGLWPRCFL IFPQLRILLL GPQESNNSTG TMWKVSALLF VLGSASLWVL AEGASTGQPE DDTETTGLEG GVAMPGAEDD VVTPGTSEDR YKSGLTTLVA TSVNS

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Diesel Particulate Matter 2.5 Induces Epithelial-to-Mesenchymal Transition and Upregulation of SARS-CoV-2 Receptor during Human Pluripotent Stem Cell-Derived Alveolar Organoid Development.

Kim JH, Kim J, Kim WJ, Choi YH, Yang SR, Hong SH
International journal of environmental research and public health 2020 Nov 13;17(22)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Effects of dPM2.5 exposure during hPSC-derived alveolar organoid (AO) development. ( A ) Schematic diagram of AO generation from hPSCs. ( B ) Representative bright field and H&E staining images of AOs. Scale bars, 100 mum. ( C ) Immunofluorescence staining for AEP (EPCAM, CPM, and HOPX, red), AEC1 (AQP5 and T1 alpha , green), AEC2 (SFTPC, red) and mesenchymal stromal cell (VIMENTIN, red) markers in AOs on day 25 of induction. Nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Scale bars, 100 mum. ( D ) qPCR analysis of the indicated AEP and AEC markers in AOs. Data are shown as fold-change relative to undifferentiated hPSCs (unhPSC). Data is presented as mean +- SD. * p < 0.05, ** p < 0.01. ( E ) Schematic diagram of dPM2.5 treatment during hPSC-derived AO development. Black lines indicate the duration of dPM2.5 treatment. Scale bars, 100 mum. ( F ) Measurement of AO size was taken by averaging the longest and shortest diameter of AOs. ( G , H ) Transcript levels of inflammation, fibrosis, and EMT-related genes were measured using qPCR. Data is presented as mean +- SD. * p < 0.05, ** p < 0.01 (vs. Control).