- MA5-16267 - Invitrogen Antibodies PDPN antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of podoplanin in lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with a Podoplanin monoclonal antibody (Product # MA5-16267) at 5 µg/mL. Goat anti-rat Ig HRP secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Podoplanin in lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Podoplanin in lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Podoplanin was achieved by transfecting NTERA-2 cl.D1 with Podoplanin specific siRNAs (Silencer® select Product # S20884, S20885). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Podoplanin knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Podoplanin Monoclonal Antibody (NZ-1.2) (Product # MA5-16267, 4 ug/mL ) and F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Podoplanin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Podoplanin Monoclonal Antibody (NZ-1.2)(Product # MA5-16267) and a 50 kDa band corresponding to Podoplanin was observed across tissues tested. Tissue extracts (30 µg lysate) of Rat testis (Lane 1), Rat Liver (Lane 2) and Rat Mammary gland (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Podoplanin in immersion fixed paraffin-embedded sections of human appendix. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 0.5 µg/mL for 1 hour at room temperature followed by the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometric analysis using Podoplanin antibody. Human NCCIT cells were probed using 0.2 µg of Podoplanin monoclonal antibody (Product # MA5-16267) (red) and 0.2 µg of isotype control antibody (green). rat PE secondary were used for this test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Podoplanin in Human NCCIT cells. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 0.2 µg followed by anti-rat PE secondary. Antibody (azide free; red) and 0.2 µg of isotype control antibody (green). Flow kit was used for this test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Podoplanin in Human NCCIT cells. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 10 µl (0.15 µg). Antibody (conjugated to Alexa Fluor (R) 488) (red) and 0.15 µg of isotype control antibody (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Podoplanin in Human NCCIT cells. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 10 µl (0.15 µg). Antibody (conjugated to Alexa Fluor (R) 647) (red) and 0.15 µg of isotype control antibody (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Podoplanin in Human NCCIT cells. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16267) using a dilution of 10 µl (0.2 µg). Antibody (PE conjugated; red) and 0.2 µg of isotype control antibody (green).

MA5-16267