Antibody data

Product number
53-6508-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
Chorionic Gonadotropin Monoclonal Antibody (FB12), Alexa Fluor™ 488, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This FB12 monoclonal antibody reacts with human Chorionic Gonadotropin (hCG). This secreted glycoprotein is composed of an alpha subunit, which is common to other peptide hormones such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), and a beta subunit which determines the specificity of the hormone. FB12 recognizes both the intact hCG (composed of the alpha and beta subunits) as well as the free beta subunit, highly expressed in both choriocarcinoma and the serum of pregnant women. hCG is normally expressed by trophoblastoid cells (syncytiotrophoblasts and extravillous cytotrophoblasts) that are responsible for maintenance of the corpus luteum during early gestation. hCG plays an important role in the regulation of angiogenesis through activation of the PI3K/mTOR pathway, resulting in upregulation of vascular endothelial growth factor (VEGF). Ectoptic expression of hCG has also been reported in multiple types of cancer (bladder, lung, breast, pancreas, renal). Applications Reported: This FB12 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F) and immunocytochemistry (ICC). Applications Tested: This FB12 antibody has been tested by immunocytochemistry on fixed BeWo cells. This can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Green dye
Isotype
IgG
Antibody clone number
FB12
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
CGB8 protein structure - 53-6508-82 shown in red.