Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 53-6508-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chorionic Gonadotropin Monoclonal Antibody (FB12), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This FB12 monoclonal antibody reacts with human Chorionic Gonadotropin (hCG). This secreted glycoprotein is composed of an alpha subunit, which is common to other peptide hormones such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), and a beta subunit which determines the specificity of the hormone. FB12 recognizes both the intact hCG (composed of the alpha and beta subunits) as well as the free beta subunit, highly expressed in both choriocarcinoma and the serum of pregnant women. hCG is normally expressed by trophoblastoid cells (syncytiotrophoblasts and extravillous cytotrophoblasts) that are responsible for maintenance of the corpus luteum during early gestation. hCG plays an important role in the regulation of angiogenesis through activation of the PI3K/mTOR pathway, resulting in upregulation of vascular endothelial growth factor (VEGF). Ectoptic expression of hCG has also been reported in multiple types of cancer (bladder, lung, breast, pancreas, renal). Applications Reported: This FB12 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F) and immunocytochemistry (ICC). Applications Tested: This FB12 antibody has been tested by immunocytochemistry on fixed BeWo cells. This can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- FB12
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry on fixed BeWo cells using 20 µg/mL Mouse IgG1 Alexa Fluor® 488 Isotype Control (left) or 20 µg/mL Anti-Human Chorionic Gonadotropin Alexa Fluor® 488 (right). Nuclei are counterstained with DAPI.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of substrate stiffness on BeWo cell beta-hCG expression. ( a , b ) BeWo cells only express beta-hCG with forskolin treatment. ( c , d ) Representative figures of cells expressing beta-hCG on soft ( c ) and stiff ( d ) substrates. Red: beta-hCG; blue: DAPI. Scale bar is 50 mum. ( e ) Ratio of BeWo cells expressing beta-hCG and ( f ) normalized media beta-hCG level when cultured on substrates with various stiffness. Data reported as mean +- standard deviation for n = 3 independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001, by two-tailed, one-way ANOVA with Holm-Sidak Post-hoc comparison.
- Conjugate
- Green dye