Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000012-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000012-M01, RRID:AB_536749
- Product name
- Mouse Monoclonal Serpin A3/alpha 1-Antichymotrypsin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SERPINA3 - serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody (1E6) [H00000012-M01] - Analysis of SERPINA3 expression in transfected 293T cell line by SERPINA3 monoclonal antibody (M01), clone 1E6.Lane 1: SERPINA3 transfected lysate(47.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody (1E6) [H00000012-M01] - Analysis of SERPINA3 transfected lysate using anti-SERPINA3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPINA3 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Serpin A3/alpha 1-Antichymotrypsin Antibody (1E6) [H00000012-M01] - Detection limit for recombinant GST tagged SERPINA3 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody (1E6) [H00000012-M01] - Analysis of monoclonal antibody to SERPINA3 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 1 ug/ml.