PA5-87710

antibody from Invitrogen Antibodies

Targeting: SERPINA3 AACT, ACT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87710

Provider

Invitrogen Antibodies

Product name

SERPINA3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HPNSPLDEEN LTQENQDRGT HVDLGLASAN VDFAFSLYKQ LVLKAPDKNV IFSPLSISTA LAFLSLGAHN TTLTEILKGL KFNLTETSEA EIHQSFQHLL RTLNQSSDEL QLSMGNAMFV KEQLSLLDRF TEDAKRLYGS EAFATDFQDS AAAKKLINDY VKNGTRGKIT DLIKDLDSQT MMVLVNYIFF KAKWEMPFDP QDTHQSRFYL SKKKWVMVPM MSLHHLTIPY FRDEELSCTV VELKYTGNAS ALFILPDQDK MEEVEAMLLP ETLKRWRDSL EFREIGELYL PKFSISRDYN LNDILLQLGI EEAFTSKADL SGITGARNLA VSQVVHKAVL DVFEEGTEAS AATAVKITLL SALVETRTIV RFNRPFLMII VPTDTQNIFF MSKVTNPKQA; Positive Samples: Mouse testis, Mouse liver, Rat liver

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.30 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using SERPINA3 Polyclonal antibody (Product # PA5-87710) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SERPINA3 in extracts of various cell lines using SERPINA3 Polyclonal Antibody (Product # PA5-87710) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells using SERPINA3 Polyclonal antibody (Product # PA5-87710). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SERPINA3 in paraffin-embedded mouse lung using SERPINA3 Polyclonal Antibody (Product # PA5-87710) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SERPINA3 in paraffin-embedded mouse lung using SERPINA3 Polyclonal Antibody (Product # PA5-87710) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SERPINA3 in paraffin-embedded rat brain using SERPINA3 Polyclonal Antibody (Product # PA5-87710) at a dilution of 1:100.