LS-C290881

antibody from LSBio

Targeting: PNP NP, PUNP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C290881

Provider

LSBio

Product name

Anti-PNP / Nucleoside Phosphorylase Antibody (aa239-289) LS-C290881

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PNP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PNP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PNP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PNP antibody used for IP at 6 ug/mg lysate. PNP was inefficiently immunoprecipitated by rabbit anti-PNP antibody BL15626. For blotting immunoprecipitated PNP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.