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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- R32597 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PNP Antibody / Purine Nucleoside Phosphorylase
- Antibody type
- Polyclonal (Antigen purified)
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Purine nucleoside phosphorylase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat thymus, 2) rat ovary, 3) mouse liver and 4) human placenta lysate using Purine nucleoside phosphorylase antibody at 0.5ug/ml. Predicted molecular weight: 32-34 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse kidney tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat kidney tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-251 MG cells with Purine Nucleoside Phosphorylase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Purine Nucleoside Phosphorylase antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U937 MG cells with Purine Nucleoside Phosphorylase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Purine Nucleoside Phosphorylase antibody.
