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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Product number
- MA5-29491 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNP Recombinant Rabbit Monoclonal Antibody (103)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human Purine nucleoside phosphorylase/PNP.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 103
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PNP Recombinant Rabbit Monoclonal Antibody (103) (Product # MA5-29491) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PNP Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg K562 Whole Cell Lysate 2 µL with PNP Recombinant Rabbit Monoclonal Antibody (103) (Product # MA5-29491) and 15 µL of 50 % Protein G agarose. Primary antibody: PNP Recombinant Rabbit Monoclonal Antibody (103), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa.
