Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AF6486 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Purine Nucleoside Phosphorylase/PNP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Purine Nucleoside Phosphorylase/PNP in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P00491
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Purine Nucleoside Phosphorylase/ PNP by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-231 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, LNCaP human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Purine Nucleoside Phosphorylase/PNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6486) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Purine Nucleoside Phosphorylase/PNP by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 36 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Purine Nucleoside Phosphorylase/PNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6486) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.