Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB6486 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Purine Nucleoside Phosphorylase/PNP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Purine Nucleoside Phosphorylase/PNP in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P00491
- Isotype
- IgG
- Antibody clone number
- 773511
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Purine Nucleoside Phosphorylase/PNP by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, Daudi human Burkitt's lymphoma cell line, HT1080 human fibrosarcoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Purine Nucleoside Phosphorylase/PNP Monoclonal Antibody (Catalog # MAB6486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Purine Nucleoside Phosphorylase/PNP in Human Liver. Purine Nucleoside Phosphorylase/PNP was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Purine Nucleoside Phosphorylase/PNP Monoclonal Antibody (Catalog # MAB6486) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.