Explore
Validate
LearnV3221
antibody from NSJ Bioreagents
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- V3221 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Alpha 1 Antitrypsin Antibody (Hepatocyte Marker)
- Antibody type
- Monoclonal
- Description
- This highly specific Alpha 1 Antitrypsin antibody is suitable for use in Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- AAT/1378
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) a partial recombinant protein, 2) human Jurkat and 3) human A549 lysate with Alpha 1 Antitrypsin antibody (clone AAT/1378). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) a partial recombinant protein, 2) human Jurkat and 3) human A549 lysate with Alpha 1 Antitrypsin antibody (clone AAT/1378). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human lung tissue lysate with Alpha 1 Antitrypsin antibody. Expected molecular weight: 47-52 kDa depending on the level of glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Alpha 1 Antitrypsin Antibody (clone AAT/1378). Confirmation of Integrity and Purity of the Antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Alpha 1 Antitrypsin Antibody (clone AAT/1378). Confirmation of Integrity and Purity of the Antibody.
