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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54354 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Protocadherin 20 Antibody / PCDH20
- Antibody type
- Polyclonal
- Description
- This highly specific Protocadherin 20 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Immunohistochemistry applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Protocadherin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) SK-BR-3, 2) SH-SY5Y, 3) MCF7, 4) HL60 and 5) rat C6 cell lysate with Protocadherin 20 antibody. Predicted molecular weight ~105 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) SK-BR-3, 2) SH-SY5Y, 3) MCF7, 4) HL60 and 5) rat brain lysate with Protocadherin 20 antibody. Predicted molecular weight ~105 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Protocadherin 20 antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human pancreas tissue with Protocadherin 20 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human MCF7 cells with Protocadherin 20 antibody; Blue=isotype control, Green= Protocadherin 20 antibody.
