Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C287012 - Provider product page
- Provider
- LSBio
- Product name
- Anti-DIAPH1 Antibody (aa1-50) LS-C287012
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Detection of Human Diaphanous 1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).