AM31730PU-N

antibody from OriGene

Targeting: CBSL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM31730PU-N

Provider

OriGene

Product name

CBSL mouse monoclonal antibody, clone 3E1, Purified

Antibody type

Monoclonal

Description

CBSL mouse monoclonal antibody, clone 3E1, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

CBSL

Isotype

IgG

Antibody clone number

3E1

Vial size

50 µg

Concentration

0.25 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of CBS expression in HeLa cell lysate.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of CBS expression in transfected 293T cell line. Lane 1: CBS transfected lysate (61 KDa). Lane 2: Non-transfected lysate.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS antibody, clone 3E1 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CBS is approximately 0.1ng/ml as a capture antibody.

Validation comment

ELISA

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation of CBS transfected lysate using anti-CBS monoclonal antibody and Protein A Magnetic Bead.

Validation comment

IP

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC