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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-22273 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CBS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, 293T, mouse liver. Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (93%), Rabbit (95%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.81 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cystathionine metabolic enzymes play a role in the inflammation resolution of human keratinocytes in response to sub-cytotoxic formaldehyde exposure.
Lee E, Kim HJ, Lee M, Jin SH, Hong SH, Ahn S, Kim SO, Shin DW, Lee ST, Noh M
Toxicology and applied pharmacology 2016 Nov 1;310:185-194
Toxicology and applied pharmacology 2016 Nov 1;310:185-194
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CBS using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CBS polyclonal antibody (Product # PA5-22273) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CBS using 30 µg 293T whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CBS polyclonal antibody (Product # PA5-22273) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CBS was performed by separating 30 µg of whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CBS Polyclonal Antibody (Product # PA5-22273) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CBS Polyclonal Antibody (Product # PA5-22273). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS Polyclonal Antibody (Product # PA5-22273) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CBS Polyclonal Antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS Polyclonal Antibody (Product # PA5-22273) diluted by 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CBS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CBS Polyclonal Antibody (Product # PA5-22273) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CBS was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: CBS Polyclonal Antibody (Product # PA5-22273) diluted at 1:200. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS (Product # PA5-22273) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CBS Polyclonal Antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 µg HepG2 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of CBS protein by 4 µg CBS Polyclonal Antibody (Product # PA5-22273). 7.5 % SDS-PAGE. The immunoprecipitated CBS protein was detected by CBS Polyclonal Antibody (Product # PA5-22273) diluted at 1:500.
