GTX26633

antibody from GeneTex

Targeting: GSTP1 FAEES3, GST3, GSTP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

GTX26633

Provider

GeneTex

Proper citation

GeneTex Cat#GTX26633, RRID:AB_10720511

Product name

GST antibody

Antibody type

Polyclonal

Antigen

Glutathione-S-Transferase [Schistosoma japonicum]

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

1,2 mg/ml

Storage

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of anti-GST antibody (GTX26633). Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 µg per lane. Primary antibody: anti-GST 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GeneTex anti-GST polyclonal antibody (GTX26633) in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 µg of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4° C. For detection DyLight™800 conjugated Donkey-a-Goat IgG was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25° C.

Validation comment

WB