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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- MAB6455 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Glutathione S-Transferase pi 1/GSTP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs and Western blots. Detects mouse and rat Glutathione S-Transferase pi 1/GSTP1 in Western Blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Mining the nucleus accumbens proteome for novel targets of alcohol self-administration in male C57BL/6J mice.
Faccidomo S, Swaim KS, Saunders BL, Santanam TS, Taylor SM, Kim M, Reid GT, Eastman VR, Hodge CW
Psychopharmacology 2018 Jun;235(6):1681-1696
Psychopharmacology 2018 Jun;235(6):1681-1696
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 26 kDa (as indicated) using 12.5 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 by Western Blot. Western blot shows lysates of human placenta tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
