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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG55372 - Provider product page
- Provider
- Arigo
- Product name
- anti-GST3 / GST pi antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 97-126 (Center) of Human GSTP1.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of K562,PC-3,Y79 cell line and mouse lung tissue lysates stained with ARG55372 anti-GST3 / GST pi antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: A549 cell stained with ARG55372 anti-GST3 / GST pi antibody (green) at 1:25 dilution, 1 h at 37°C. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG55372 anti-GST3 / GST pi antibody. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). GSTP1 immunoreactivity is localized to Cytoplasm and Mitochondria significantly.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: A549 cell stained with ARG55372 anti-GST3 / GST pi antibody (green) at 1:25 dilution, 1 h at 37°C. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG55372 anti-GST3 / GST pi antibody. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). GSTP1 immunoreactivity is localized to Cytoplasm significantly.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin fixed and paraffin embedded human colon carcinoma stained with ARG55372 anti-GST3 / GST pi antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: Ramos cells stained with ARG55372 anti-GST3 / GST pi antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
