MA5-15309

antibody from Invitrogen Antibodies

Targeting: GSTP1 FAEES3, GST3, GSTP

Western blot

ELISA

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: MA5-15309 - Provider product page
Provider: Invitrogen Antibodies
Product name: GSTP1 Monoclonal Antibody (3F2C2)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15309 targets GSTP1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15309 immunogen is purified recombinant fragment of human GSTP1 expressed in E. Coli. MA5-15309 detects GSTP1 which has a predicted molecular weight of approximately 23kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 3F2C2
Vial size: 100 µL
Concentration: Conc. not determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GSTP1 protein structure - MA5-15309 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HepG2 (left) and L-02 (right) cells using GSTP1 monoclonal antibody (Product # MA5-15309) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PC-3 cells using GSTP1 monoclonal antibody (Product # MA5-15309) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HepG2 (left) and L-02 (right) cells using GSTP1 monoclonal antibody (Product # MA5-15309) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Glutathione S-transferase P was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GSTP1 Monoclonal Antibody (3F2C2) (Product # MA5-15309) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic and Nuclear localization. Panel e represents LNCaP cells having no expression of GSTP1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.