Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29558 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSTP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HCT116, Mouse brain, Rat brain. Predicted reactivity: Mouse (85%), Rat (85%), Dog (86%), Rhesus Monkey (97%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.64 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proteomics signature of autoimmune atrophic gastritis: towards a link with gastric cancer.
DCAF1 regulates Treg senescence via the ROS axis during immunological aging.
Repetto O, De Re V, Giuffrida P, Lenti MV, Magris R, Venerito M, Steffan A, Di Sabatino A, Cannizzaro R
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2021 May;24(3):666-679
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2021 May;24(3):666-679
DCAF1 regulates Treg senescence via the ROS axis during immunological aging.
Guo Z, Wang G, Wu B, Chou WC, Cheng L, Zhou C, Lou J, Wu D, Su L, Zheng J, Ting JP, Wan YY
The Journal of clinical investigation 2020 Nov 2;130(11):5893-5908
The Journal of clinical investigation 2020 Nov 2;130(11):5893-5908
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSTP1 using 30 µg of HCT116 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a GSTP1 polyclonal antibody (Product # PA5-29558) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GSTP1 was performed by separating 30 µg of whole cell lysates by 12 % SDS-PAGE. Proteins were transferred to a membrane and probed with a GSTP1 Polyclonal Antibody (Product # PA5-29558) at a dilution of 1:5000. A. A549, B. HCT116.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GSTP1 Polyclonal Antibody (Product # PA5-29558). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. GSTP1 Polyclonal Antibody (Product # PA5-29558) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTP1 Polyclonal Antibody detects GSTP1 protein by western blot analysis. A. 50 µg Rat brain lysate/extract.12% SDS-PAGE. GSTP1 Polyclonal Antibody (Product # PA5-29558) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-GSTP1 Polyclonal Antibody (Product # PA5-29558) and a 23 kDa band corresponding to Glutathione S-transferase P was observed in PC-3, HeLa, MOLT-4, HT-29, MCF 10A and MDA-MB-231 which are reported to be positive and not in any other cell lines. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), LNCaP (Lane 2), HeLa (Lane 3), MOLT-4 (Lane 4), HT-29 (Lane 5), MCF 10A (Lane 6), MDA-MB-231 (Lane 7), MCF7 (Lane 8) and Hep G2 (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GSTP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GSTP1 Polyclonal Antibody (Product # PA5-29558) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GSTP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GSTP1 Polyclonal Antibody (Product # PA5-29558) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTP1 Polyclonal Antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. GSTP1 Polyclonal Antibody (Product # PA5-29558) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTP1 Polyclonal Antibody detects GSTP1 protein at nucleus on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. GSTP1 Polyclonal Antibody (Product # PA5-29558) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTP1 Polyclonal Antibody detects GSTP1 protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GSTP1 Polyclonal Antibody (Product # PA5-29558) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GSTP1 Polyclonal Antibody detects GSTP1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. GSTP1 Polyclonal Antibody (Product # PA5-29558) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using GSTP1 (Product # PA5-29558) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Immunochemical detection of seven differentially abundant proteins, indicated by gene names on the right. Samples are pools of extracted protein from six gastric biopsy specimens per study group. a Image of the gel acquired with Chemidoc system before transfer to nitrocellulose membranes. b Western blots of the selected proteins
