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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-510-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-510-C100, RRID:AB_2051525
- Product name
- Glutathione S-transferase-Pi monoclonal antibody (USal-hGST-Pi-McAb-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- USal-hGST-Pi-McAb-1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Bile acid secretion during rat liver carcinogenesis.
Monte MJ, Palomero F, Sainz GR, Domínguez M, Díez M, Toraño A, Marin JJ
Life sciences 2000 Feb 11;66(12):1085-95
Life sciences 2000 Feb 11;66(12):1085-95
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- ÊLeft: Polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue staining of molecular weight standard (MW), pure GST-Pi protein and liver homogenate (5µg protein) from normal rat liver (Control) or from liver tissue containing neoplastic lesions at different time points during hepatocarcinogenesis. Right: Western blot using anti-GST-Pi MAb.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry of rat liver at 12, 20 or 32 weeks after initiation of liver carcinogenesis. GST-Pi-positive tissue was stained with an anti-GST-Pi MAb in a 30µm thick rat liver slice. Original magnification 4x.
