Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX105970 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105970, RRID:AB_1950856
- Product name
- Monoamine Oxidase B antibody [N2C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references α-Synuclein stimulation of monoamine oxidase-B and legumain protease mediates the pathology of Parkinson's disease.
Kang SS, Ahn EH, Zhang Z, Liu X, Manfredsson FP, Sandoval IM, Dhakal S, Iuvone PM, Cao X, Ye K
The EMBO journal 2018 Jun 15;37(12)
The EMBO journal 2018 Jun 15;37(12)
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Supportive validation
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- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse liver 7.5% SDS PAGE GTX105970 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
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- Experimental details
- Sample(30 ?g of whole cell lysate)A:Hep G2(GTX27900)7.5% SDS PAGEGTX105970 diluted at 1:1500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:1500.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ELISA detection of MAOB using GTX105970 for capture at a concentration of 2.5 ?g/mL and GTX89368 for detection at a concentration of 0.5 ?g/mL.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: Monoamine Oxidase B protein stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Monoamine Oxidase B(GTX105970) antibody at 1:100 dilution.
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- GeneTex (provider)
- Main image
- Experimental details
- Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.