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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-59569 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-59569, RRID:AB_11031159
- Product name
- Rabbit Polyclonal Monoamine Oxidase B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Amphetamine manipulates monoamine oxidase-A level and behavior using theranostic aptamers of transcription factors AP-1/NF-kB.
Liu CH, Ren J, Liu PK
Journal of biomedical science 2016 Feb 3;23:21
Journal of biomedical science 2016 Feb 3;23:21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Human heart.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Mice were pretreated with 5ECdsAP1 aptamer before amphetamine application; tissue samples collected (n = 4 per group) from the VTA at 90 (60 + 30 min) and 180 (60 + 30 + 90 min) minutes after amphetamine. Protein was obtained for Western blot quantitation of MAO-A level using Actin (lower molecular fragments) as a reference shown in lanes 2-4 (90 min samples) or lane 5-6 (180 min). Total protein (10 ug) was used for all lanes except lanes 7 & 9. The blot was stripped and used for MAO-B. Lane 1: molecular size marker of 10 KD ladder; lane 2: 5ECdsAP1 (4 nmol/kg, icv, 1/2 dose); lanes 3 & 5: saline (2 ul, icv); lanes 4 & 6: 5ECdsAP1 (8 nmol/kg, icv); lanes 7, 8, 9 are controls of naive mice with no aptamer or amphetamine (5, 10 and 20 ug protein). Image collected and cropped by CiteAb from the following publication (http://www.jbiomedsci.com/content/23/1/21), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-59569] - Human Liver tissue.
