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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28338 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Monoamine Oxidase B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse liver.
- Concentration
- 0.88 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Monoamine Oxidase B using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Monoamine Oxidase B polyclonal antibody (Product # PA5-28338) at a dilution of 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) and a 58kDa band corresponding to Amine oxidase [flavin-containing] B was observed across the cell lines tested and tissues tested. Whole cell extracts (30 µg lysate) of U2OS (Lane 1), Hep G2 (Lane 2), SH-SY5Y (Lane 3), Jurkat (Lane 4), SK-OV-3 (Lane 5), Mouse Liver (Lane 6), Rat Liver (Lane 7), Mouse Testis (Lane 8), Rat Testis (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338). Sample (50 µg of whole cell lysate). Lane A: Mouse liver. 7.5% SDS PAGE. Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338). HepG2 and mitochondria extracts (30 µg) were separated by SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Monoamine Oxidase B was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) at a dilution of 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) and a 58kDa band corresponding to Amine oxidase [flavin-containing] B was observed across the cell lines tested and tissues tested. Whole cell extracts (30 µg lysate) of U2OS (Lane 1), Hep G2 (Lane 2), SH-SY5Y (Lane 3), Jurkat (Lane 4), SK-OV-3 (Lane 5), Mouse Liver (Lane 6), Rat Liver (Lane 7), Mouse Testis (Lane 8), Rat Testis (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of Monoamine Oxidase B using a MAOB polyclonal antibody (Product # PA5-28338) for capture at a concentration of 2.5 µg/mL, and an alternate MAOB antibody for detection at a concentration of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Monoamine Oxidase B was performed in HepG2 cells fixed in ice cold MeOH for 5 min. Green: Monoamine Oxidase B Polyclonal Antibody (Product # PA5 28338) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Monoamine Oxidase B was performed in paraffin-embedded mouse lung tissue using Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Monoamine Oxidase B was performed in paraffin-embedded mouse liver tissue using Monoamine Oxidase B Polyclonal Antibody (Product # PA5-28338) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
