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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29824 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Monoamine Oxidase B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, Rat liver. Predicted reactivity: Human (98%), Mouse (89%), Rat (88%), Dog (94%), Pig (90%), Bovine (90%), Guinea pig (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Monoamine Oxidase B in Rat tissue extract (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with Monoamine Oxidase B Polyclonal antibody (Product # PA5-29824) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824) and a 58kDa band corresponding to Amine oxidase [flavin-containing] B was observed across the cell lines tested and tissues tested with non specific bands(*). Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), SH-SY5Y (Lane 2), Jurkat (Lane 3), SK-OV-3 (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 7.5% SDS PAGE. Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824). Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824) and a 58kDa band corresponding to Amine oxidase [flavin-containing] B was observed across the cell lines tested and tissues tested with non specific bands(*). Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), SH-SY5Y (Lane 2), Jurkat (Lane 3), SK-OV-3 (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Monoamine Oxidase B in methanol-fixed HepG2 cells using a Monoamine Oxidase B polyclonal antibody (Product # PA5-29824) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Amine oxidase [flavin-containing] B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Monoamine Oxidase B Polyclonal Antibody (Product # PA5-29824) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nucleus and cytoplasm localization. Panel e represents SK-OV-3. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Monoamine Oxidase B (Product # PA5-29824) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
