NB100-2826

antibody from Novus Biologicals

Targeting: MAOB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2826

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2826, RRID:AB_2297086

Product name

Goat Polyclonal Monoamine Oxidase B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is not expected to cross-react with MAOA.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Association analysis of the monoamine oxidase A and B genes with attention deficit hyperactivity disorder (ADHD) in an Irish sample: preferential transmission of the MAO-A 941G allele to affected children.

Domschke K, Sheehan K, Lowe N, Kirley A, Mullins C, O'sullivan R, Freitag C, Becker T, Conroy J, Fitzgerald M, Gill M, Hawi Z
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 2005 Apr 5;134B(1):110-4

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Western blot analysis at 1 ug/ml in Human Brain lysate (35ug protein in RIPA buffer).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Human Liver (A) with antibody at 0.1 ug/mL and Testes (B) and Breast Cancer (C) lysate with antibody at 0.3ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Monoamine Oxidase B Antibody [NB100-2826] - (0.5ug/ml) as the reporter with capture rabbit antibody (2.5ug/ml).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.