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- Antibody Data
- Antigen structure
- References [95]
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- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- 14626-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14626-1-AP, RRID:AB_2187734
- Product name
- SHARPIN antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SHARPIN antibody (Cat. #14626-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CRISPR/Cas9-mediated knock-in cells of the late-onset Alzheimer's disease-risk variant, SHARPIN G186R, reveal reduced NF-κB pathway and accelerated Aβ secretion.
Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency.
Focal adhesions contain three specialized actin nanoscale layers.
RNF31 promotes proliferation and invasion of hepatocellular carcinoma via nuclear factor kappaB activation.
LUBAC promotes angiogenesis and lung tumorigenesis by ubiquitinating and antagonizing autophagic degradation of HIF1α.
P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability.
Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.
HOIL1 mediates MDA5 activation through ubiquitination of LGP2.
Linear ubiquitination improves NFAT1 protein stability and facilitates NFAT1 signalling in Kawasaki disease.
Characterization of SHARPIN knockout Syrian hamsters developed using CRISPR/Cas9 system.
Myeloid OTULIN deficiency couples RIPK3-dependent cell death to Nlrp3 inflammasome activation and IL-1β secretion.
TSG101 Physically Interacts with Linear Ubiquitin Chain Assembly Complex (LUBAC) and Upregulates the TNFα-Induced NF-κB Activation.
SPATA2 restricts OTULIN-dependent LUBAC activity independently of CYLD.
Mechanistic insights into the homo-dimerization of HOIL-1L and SHARPIN.
OTULIN protects the intestinal epithelium from apoptosis during inflammation and infection.
PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIP(L) to promote TNFα-mediated cell death.
Linear ubiquitination of PTEN impairs its function to promote prostate cancer progression.
Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development.
The FDA-approved drug Auranofin has a dual inhibitory effect on SARS-CoV-2 entry and NF-κB signaling.
SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis.
Identification and molecular analysis of RNF31 Q622H germline polymorphism.
HOIP modulates the stability of GPx4 by linear ubiquitination.
SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation.
Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORAS.
Human ZBP1 induces cell death-independent inflammatory signaling via RIPK3 and RIPK1.
A20 controls RANK-dependent osteoclast formation and bone physiology.
Linear ubiquitination modification of NR6A1 by LUBAC inhibits RIPK3 kinase activity and attenuates apoptosis of vascular smooth muscle cells.
Crosstalk Between NDP52 and LUBAC in Innate Immune Responses, Cell Death, and Xenophagy.
Hypoxia regulates overall mRNA homeostasis by inducing Met(1)-linked linear ubiquitination of AGO2 in cancer cells.
SHARPIN regulates the development of clear cell renal cell carcinoma by promoting von Hippel-Lindau protein ubiquitination and degradation.
OTULIN maintains skin homeostasis by controlling keratinocyte death and stem cell identity.
Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains.
Primidone blocks RIPK1-driven cell death and inflammation.
Impaired RIPK1 ubiquitination sensitizes mice to TNF toxicity and inflammatory cell death.
SHARPIN stabilizes β-catenin through a linear ubiquitination-independent manner to support gastric tumorigenesis.
T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.
A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.
OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.
Human TBK1 deficiency leads to autoinflammation driven by TNF-induced cell death.
Cellular and Mathematical Analyses of LUBAC Involvement in T Cell Receptor-Mediated NF-κB Activation Pathway.
Systematic analysis of the IL-17 receptor signalosome reveals a robust regulatory feedback loop.
Subquinocin, a small molecule inhibitor of CYLD and USP-family deubiquitinating enzymes, promotes NF-κB signaling.
RIPK1-mediated immunogenic cell death promotes anti-tumour immunity against soft-tissue sarcoma.
The parkin-coregulated gene product PACRG promotes TNF signaling by stabilizing LUBAC.
OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners.
Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses.
Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation.
The anticonvulsive Phenhydan(®) suppresses extrinsic cell death.
A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.
MST1 Negatively Regulates TNFα-Induced NF-κB Signaling through Modulating LUBAC Activity.
Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.
Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation.
LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L.
LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.
Parkin regulates NF-κB by mediating site-specific ubiquitination of RIPK1.
OTULIN limits cell death and inflammation by deubiquitinating LUBAC.
ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC.
Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2.
HOIL1 Is Essential for the Induction of Type I and III Interferons by MDA5 and Regulates Persistent Murine Norovirus Infection.
Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice.
The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex.
A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.
A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN.
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death.
Decreased linear ubiquitination of NEMO and FADD on apoptosis with caspase-mediated cleavage of HOIP.
MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death.
SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitination.
HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains.
SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland.
Lack of interaction between NEMO and SHARPIN impairs linear ubiquitination and NF-κB activation and leads to incontinentia pigmenti.
Roles of the TRAF6 and Pellino E3 ligases in MyD88 and RANKL signaling.
SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death.
--LUBAC deficiency perturbs TLR3 signaling to cause immunodeficiency and autoinflammation.
An Essential Role for SHARPIN in the Regulation of Caspase 1 Activity in Sepsis.
CYLD Limits Lys63- and Met1-Linked Ubiquitin at Receptor Complexes to Regulate Innate Immune Signaling.
Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling.
SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis.
The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice.
Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination.
Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia.
LUBAC-Recruited CYLD and A20 Regulate Gene Activation and Cell Death by Exerting Opposing Effects on Linear Ubiquitin in Signaling Complexes.
HOIP deficiency causes embryonic lethality by aberrant TNFR1-mediated endothelial cell death.
Binding of OTULIN to the PUB domain of HOIP controls NF-κB signaling.
Molecular basis and regulation of OTULIN-LUBAC interaction.
TNFR1-dependent cell death drives inflammation in Sharpin-deficient mice.
Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.
OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1-linked polyubiquitin.
OTULIN restricts Met1-linked ubiquitination to control innate immune signaling.
Disease-causing mutations in the XIAP BIR2 domain impair NOD2-dependent immune signalling.
The ubiquitin ligase XIAP recruits LUBAC for NOD2 signaling in inflammation and innate immunity.
Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency.
Asanomi Y, Kimura T, Shimoda N, Shigemizu D, Niida S, Ozaki K
Journal of human genetics 2024 May;69(5):171-176
Journal of human genetics 2024 May;69(5):171-176
Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency.
Oda H, Manthiram K, Chavan PP, Rieser E, Veli Ö, Kaya Ö, Rauch C, Nakabo S, Kuehn HS, Swart M, Wang Y, Çelik NI, Molitor A, Ziaee V, Movahedi N, Shahrooei M, Parvaneh N, Alipour-Olyei N, Carapito R, Xu Q, Preite S, Beck DB, Chae JJ, Nehrebecky M, Ombrello AK, Hoffmann P, Romeo T, Deuitch NT, Matthíasardóttir B, Mullikin J, Komarow H, Stoddard J, Niemela J, Dobbs K, Sweeney CL, Anderton H, Lawlor KE, Yoshitomi H, Yang D, Boehm M, Davis J, Mudd P, Randazzo D, Tsai WL, Gadina M, Kaplan MJ, Toguchida J, Mayer CT, Rosenzweig SD, Notarangelo LD, Iwai K, Silke J, Schwartzberg PL, Boisson B, Casanova JL, Bahram S, Rao AP, Peltzer N, Walczak H, Lalaoui N, Aksentijevich I, Kastner DL
Nature immunology 2024 May;25(5):764-777
Nature immunology 2024 May;25(5):764-777
Focal adhesions contain three specialized actin nanoscale layers.
Kumari R, Ven K, Chastney M, Kokate SB, Peränen J, Aaron J, Kogan K, Almeida-Souza L, Kremneva E, Poincloux R, Chew TL, Gunning PW, Ivaska J, Lappalainen P
Nature communications 2024 Mar 21;15(1):2547
Nature communications 2024 Mar 21;15(1):2547
RNF31 promotes proliferation and invasion of hepatocellular carcinoma via nuclear factor kappaB activation.
Hoshino K, Nakazawa S, Yokobori T, Hagiwara K, Ishii N, Tsukagoshi M, Igarashi T, Araki K, Harimoto N, Tokunaga F, Shirabe K
Scientific reports 2024 Jan 3;14(1):346
Scientific reports 2024 Jan 3;14(1):346
LUBAC promotes angiogenesis and lung tumorigenesis by ubiquitinating and antagonizing autophagic degradation of HIF1α.
Jin Y, Peng Y, Xu J, Yuan Y, Yang N, Zhang Z, Xu L, Li L, Xiong Y, Sun D, Pan Y, Wu R, Fu J
Oncogenesis 2024 Jan 25;13(1):6
Oncogenesis 2024 Jan 25;13(1):6
P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability.
Zheng Z, Shang X, Sun K, Hou Y, Zhang X, Xu J, Liu H, Ruan Z, Hou L, Guo Z, Wang G, Xu F, Guo F
Free radical biology & medicine 2024 Feb 20;212:336-348
Free radical biology & medicine 2024 Feb 20;212:336-348
Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.
Fu Y, Li L, Zhang X, Deng Z, Wu Y, Chen W, Liu Y, He S, Wang J, Xie Y, Tu Z, Lyu Y, Wei Y, Wang S, Cui CP, Liu CH, Zhang L
Nature communications 2024 Apr 6;15(1):2974
Nature communications 2024 Apr 6;15(1):2974
HOIL1 mediates MDA5 activation through ubiquitination of LGP2.
Cheng D, Zhu J, Liu G, Gack MU, MacDuff DA
bioRxiv : the preprint server for biology 2024 Apr 3;
bioRxiv : the preprint server for biology 2024 Apr 3;
Linear ubiquitination improves NFAT1 protein stability and facilitates NFAT1 signalling in Kawasaki disease.
Miao Y, Qian G, Zhang R, Yuan Y, Zuo Y, Ding Y, Li X, Tang Y, Zheng H, Lv H
The FEBS journal 2023 Sep;290(17):4224-4237
The FEBS journal 2023 Sep;290(17):4224-4237
Characterization of SHARPIN knockout Syrian hamsters developed using CRISPR/Cas9 system.
Miao J, Lan T, Guo H, Wang J, Zhang G, Wang Z, Yang P, Li H, Zhang C, Wang Y, Li XM, Miao M
Animal models and experimental medicine 2023 Oct;6(5):489-498
Animal models and experimental medicine 2023 Oct;6(5):489-498
Myeloid OTULIN deficiency couples RIPK3-dependent cell death to Nlrp3 inflammasome activation and IL-1β secretion.
Doglio MG, Verboom L, Ruilova Sosoranga E, Frising UC, Asaoka T, Gansemans Y, Van Nieuwerburgh F, van Loo G, Wullaert A
Science immunology 2023 Nov 24;8(89):eadf4404
Science immunology 2023 Nov 24;8(89):eadf4404
TSG101 Physically Interacts with Linear Ubiquitin Chain Assembly Complex (LUBAC) and Upregulates the TNFα-Induced NF-κB Activation.
Kim E, Cho H, Lee G, Baek H, Lee IY, Choi EJ
Molecules and cells 2023 Jul 31;46(7):430-440
Molecules and cells 2023 Jul 31;46(7):430-440
SPATA2 restricts OTULIN-dependent LUBAC activity independently of CYLD.
Griewahn L, Müller-Foxworth M, Peintner L, Wissler M, Weiss M, Brauns-Schubert P, Massoumi R, Borner C, Groß O, Yabal M, Charvet C, Maurer U
Cell reports 2023 Jan 31;42(1):111961
Cell reports 2023 Jan 31;42(1):111961
Mechanistic insights into the homo-dimerization of HOIL-1L and SHARPIN.
Zhang Y, Xu X, Wang Y, Wang Y, Zhou X, Pan L
Biochemical and biophysical research communications 2023 Dec 31;689:149239
Biochemical and biophysical research communications 2023 Dec 31;689:149239
OTULIN protects the intestinal epithelium from apoptosis during inflammation and infection.
Verboom L, Anderson CJ, Jans M, Petta I, Blancke G, Martens A, Sze M, Hochepied T, Ravichandran KS, Vereecke L, van Loo G
Cell death & disease 2023 Aug 19;14(8):534
Cell death & disease 2023 Aug 19;14(8):534
PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIP(L) to promote TNFα-mediated cell death.
Wu G, Li D, Liang W, Sun W, Xie X, Tong Y, Shan B, Zhang M, Lu X, Yuan J, Li Y
Cell death & disease 2022 Sep 7;13(9):773
Cell death & disease 2022 Sep 7;13(9):773
Linear ubiquitination of PTEN impairs its function to promote prostate cancer progression.
Guo Y, He J, Zhang H, Chen R, Li L, Liu X, Huang C, Qiang Z, Zhou Z, Wang Y, Huang J, Zhao X, Zheng J, Chen GQ, Yu J
Oncogene 2022 Oct;41(44):4877-4892
Oncogene 2022 Oct;41(44):4877-4892
Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development.
Li M, Liu Y, Xu C, Zhao Q, Liu J, Xing M, Li X, Zhang H, Wu X, Wang L, Ou Y, Wu X, Zhao X, Liu H, Qiu L, Li F, Li J, Rong W, Luo Y, Deng J, Wang X, Wang Z, Zhao Y, Lv A, Li Q, Zhang H
Cell death and differentiation 2022 Oct;29(10):2034-2045
Cell death and differentiation 2022 Oct;29(10):2034-2045
The FDA-approved drug Auranofin has a dual inhibitory effect on SARS-CoV-2 entry and NF-κB signaling.
Laplantine E, Chable-Bessia C, Oudin A, Swain J, Soria A, Merida P, Gourdelier M, Mestiri S, Besseghe I, Bremaud E, Neyret A, Lyonnais S, Favard C, Benaroch P, Hubert M, Schwartz O, Guerin M, Danckaert A, Del Nery E, Muriaux D, Weil R
iScience 2022 Oct 21;25(10):105066
iScience 2022 Oct 21;25(10):105066
SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis.
Butt U, Khan MH, Pouwels J, Westermarck J
Journal of cell science 2022 Oct 15;135(20)
Journal of cell science 2022 Oct 15;135(20)
Identification and molecular analysis of RNF31 Q622H germline polymorphism.
Nakazawa S, Mamiya R, Kawabata-Iwakawa R, Oikawa D, Kaira K, Tokunaga F, Nobusawa S, Sato Y, Sasaki A, Yajima T, Shirabe K
Oncology letters 2022 Nov;24(5):394
Oncology letters 2022 Nov;24(5):394
HOIP modulates the stability of GPx4 by linear ubiquitination.
Dong K, Wei R, Jin T, Zhang M, Shen J, Xiang H, Shan B, Yuan J, Li Y
Proceedings of the National Academy of Sciences of the United States of America 2022 Nov;119(44):e2214227119
Proceedings of the National Academy of Sciences of the United States of America 2022 Nov;119(44):e2214227119
SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation.
Yan L, Zhang T, Wang K, Chen Z, Yang Y, Shan B, Sun Q, Zhang M, Zhang Y, Zhong Y, Liu N, Gu J, Xu D
Nature communications 2022 Nov 22;13(1):7153
Nature communications 2022 Nov 22;13(1):7153
Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORAS.
Zinngrebe J, Moepps B, Monecke T, Gierschik P, Schlichtig F, Barth TFE, Strauß G, Boldrin E, Posovszky C, Schulz A, Beringer O, Rieser E, Jacobsen EM, Lorenz MR, Schwarz K, Pannicke U, Walczak H, Niessing D, Schuetz C, Fischer-Posovszky P, Debatin KM
EMBO molecular medicine 2022 Mar 7;14(3):e14901
EMBO molecular medicine 2022 Mar 7;14(3):e14901
Human ZBP1 induces cell death-independent inflammatory signaling via RIPK3 and RIPK1.
Peng R, Wang CK, Wang-Kan X, Idorn M, Kjaer M, Zhou FY, Fiil BK, Timmermann F, Orozco SL, McCarthy J, Leung CS, Lu X, Bagola K, Rehwinkel J, Oberst A, Maelfait J, Paludan SR, Gyrd-Hansen M
EMBO reports 2022 Dec 6;23(12):e55839
EMBO reports 2022 Dec 6;23(12):e55839
A20 controls RANK-dependent osteoclast formation and bone physiology.
Martens A, Hertens P, Priem D, Rinotas V, Meletakos T, Gennadi M, Van Hove L, Louagie E, Coudenys J, De Muynck A, Gaublomme D, Sze M, van Hengel J, Catrysse L, Hoste E, Zajac JD, Davey RA, Van Hoorebeke L, Hochepied T, Bertrand MJM, Armaka M, Elewaut D, van Loo G
EMBO reports 2022 Dec 6;23(12):e55233
EMBO reports 2022 Dec 6;23(12):e55233
Linear ubiquitination modification of NR6A1 by LUBAC inhibits RIPK3 kinase activity and attenuates apoptosis of vascular smooth muscle cells.
Cai Z, He X, Liu S, Bai Y, Pan B, Wu K
Journal of biochemical and molecular toxicology 2022 Aug;36(8):e23091
Journal of biochemical and molecular toxicology 2022 Aug;36(8):e23091
Crosstalk Between NDP52 and LUBAC in Innate Immune Responses, Cell Death, and Xenophagy.
Miyashita H, Oikawa D, Terawaki S, Kabata D, Shintani A, Tokunaga F
Frontiers in immunology 2021;12:635475
Frontiers in immunology 2021;12:635475
Hypoxia regulates overall mRNA homeostasis by inducing Met(1)-linked linear ubiquitination of AGO2 in cancer cells.
Zhang H, Zhao X, Guo Y, Chen R, He J, Li L, Qiang Z, Yang Q, Liu X, Huang C, Lu R, Fang J, Cao Y, Huang J, Wang Y, Huang J, Chen GQ, Cheng J, Yu J
Nature communications 2021 Sep 13;12(1):5416
Nature communications 2021 Sep 13;12(1):5416
SHARPIN regulates the development of clear cell renal cell carcinoma by promoting von Hippel-Lindau protein ubiquitination and degradation.
Yin R, Liu S
Cancer science 2021 Oct;112(10):4100-4111
Cancer science 2021 Oct;112(10):4100-4111
OTULIN maintains skin homeostasis by controlling keratinocyte death and stem cell identity.
Hoste E, Lecomte K, Annusver K, Vandamme N, Roels J, Maschalidi S, Verboom L, Vikkula HK, Sze M, Van Hove L, Verstaen K, Martens A, Hochepied T, Saeys Y, Ravichandran K, Kasper M, van Loo G
Nature communications 2021 Oct 8;12(1):5913
Nature communications 2021 Oct 8;12(1):5913
Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains.
Elliott PR, Leske D, Wagstaff J, Schlicher L, Berridge G, Maslen S, Timmermann F, Ma B, Fischer R, Freund SMV, Komander D, Gyrd-Hansen M
Cell reports 2021 Oct 5;37(1):109777
Cell reports 2021 Oct 5;37(1):109777
Primidone blocks RIPK1-driven cell death and inflammation.
Riebeling T, Jamal K, Wilson R, Kolbrink B, von Samson-Himmelstjerna FA, Moerke C, Ramos Garcia L, Dahlke E, Michels F, Lühder F, Schunk D, Doldi P, Tyczynski B, Kribben A, Flüh C, Theilig F, Kunzendorf U, Meier P, Krautwald S
Cell death and differentiation 2021 May;28(5):1610-1626
Cell death and differentiation 2021 May;28(5):1610-1626
Impaired RIPK1 ubiquitination sensitizes mice to TNF toxicity and inflammatory cell death.
Kist M, Kőműves LG, Goncharov T, Dugger DL, Yu C, Roose-Girma M, Newton K, Webster JD, Vucic D
Cell death and differentiation 2021 Mar;28(3):985-1000
Cell death and differentiation 2021 Mar;28(3):985-1000
SHARPIN stabilizes β-catenin through a linear ubiquitination-independent manner to support gastric tumorigenesis.
Zhang L, Liu Q, Liu KW, Qin ZY, Zhu GX, Shen LT, Zhang N, Liu BY, Che LR, Li JY, Wang T, Wen LZ, Liu KJ, Guo Y, Yin XR, Wang XW, Zhou ZH, Xiao HL, Cui YH, Bian XW, Lan CH, Chen D, Wang B
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2021 Mar;24(2):402-416
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2021 Mar;24(2):402-416
T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.
Chun N, Ang RL, Chan M, Fairchild RL, Baldwin WM 3rd, Horwitz JK, Gelles JD, Chipuk JE, Kelliher MA, Pavlov VI, Li Y, Homann D, Heeger PS, Ting AT
JCI insight 2021 Dec 22;6(24)
JCI insight 2021 Dec 22;6(24)
A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.
Zhang Y, Huang K, Zhang Y, Han T, Li L, Ruan C, Sun YH, Shi W, Han W, Wu SQ, Song J, Liu J, Han J
PLoS biology 2021 Aug;19(8):e3001304
PLoS biology 2021 Aug;19(8):e3001304
OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.
Fu Y, Wang H, Dai H, Zhu Q, Cui CP, Sun X, Li Y, Deng Z, Zhou X, Ge Y, Peng Z, Yuan C, Wu B, Yang X, Li R, Liu CH, He F, Wei W, Zhang L
Molecular cell 2021 Aug 5;81(15):3187-3204.e7
Molecular cell 2021 Aug 5;81(15):3187-3204.e7
Human TBK1 deficiency leads to autoinflammation driven by TNF-induced cell death.
Taft J, Markson M, Legarda D, Patel R, Chan M, Malle L, Richardson A, Gruber C, Martín-Fernández M, Mancini GMS, van Laar JAM, van Pelt P, Buta S, Wokke BHA, Sabli IKD, Sancho-Shimizu V, Chavan PP, Schnappauf O, Khubchandani R, Cüceoğlu MK, Özen S, Kastner DL, Ting AT, Aksentijevich I, Hollink IHIM, Bogunovic D
Cell 2021 Aug 19;184(17):4447-4463.e20
Cell 2021 Aug 19;184(17):4447-4463.e20
Cellular and Mathematical Analyses of LUBAC Involvement in T Cell Receptor-Mediated NF-κB Activation Pathway.
Oikawa D, Hatanaka N, Suzuki T, Tokunaga F
Frontiers in immunology 2020;11:601926
Frontiers in immunology 2020;11:601926
Systematic analysis of the IL-17 receptor signalosome reveals a robust regulatory feedback loop.
Draberova H, Janusova S, Knizkova D, Semberova T, Pribikova M, Ujevic A, Harant K, Knapkova S, Hrdinka M, Fanfani V, Stracquadanio G, Drobek A, Ruppova K, Stepanek O, Draber P
The EMBO journal 2020 Sep 1;39(17):e104202
The EMBO journal 2020 Sep 1;39(17):e104202
Subquinocin, a small molecule inhibitor of CYLD and USP-family deubiquitinating enzymes, promotes NF-κB signaling.
Yamanaka S, Sato Y, Oikawa D, Goto E, Fukai S, Tokunaga F, Takahashi H, Sawasaki T
Biochemical and biophysical research communications 2020 Mar 26;524(1):1-7
Biochemical and biophysical research communications 2020 Mar 26;524(1):1-7
RIPK1-mediated immunogenic cell death promotes anti-tumour immunity against soft-tissue sarcoma.
Smith HG, Jamal K, Dayal JH, Tenev T, Kyula-Currie J, Guppy N, Gazinska P, Roulstone V, Liccardi G, Davies E, Roxanis I, Melcher AA, Hayes AJ, Inman GJ, Harrington KJ, Meier P
EMBO molecular medicine 2020 Jun 8;12(6):e10979
EMBO molecular medicine 2020 Jun 8;12(6):e10979
The parkin-coregulated gene product PACRG promotes TNF signaling by stabilizing LUBAC.
Meschede J, Šadić M, Furthmann N, Miedema T, Sehr DA, Müller-Rischart AK, Bader V, Berlemann LA, Pilsl A, Schlierf A, Barkovits K, Kachholz B, Rittinger K, Ikeda F, Marcus K, Schaefer L, Tatzelt J, Winklhofer KF
Science signaling 2020 Feb 4;13(617)
Science signaling 2020 Feb 4;13(617)
OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Verboom L, Martens A, Priem D, Hoste E, Sze M, Vikkula H, Van Hove L, Voet S, Roels J, Maelfait J, Bongiovanni L, de Bruin A, Scott CL, Saeys Y, Pasparakis M, Bertrand MJM, van Loo G
Cell reports 2020 Feb 18;30(7):2237-2247.e6
Cell reports 2020 Feb 18;30(7):2237-2247.e6
Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners.
Li X, Zhang M, Huang X, Liang W, Li G, Lu X, Li Y, Pan H, Shi L, Zhu H, Qian L, Shan B, Yuan J
Nature communications 2020 Dec 11;11(1):6364
Nature communications 2020 Dec 11;11(1):6364
Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses.
Oikawa D, Sato Y, Ohtake F, Komakura K, Hanada K, Sugawara K, Terawaki S, Mizukami Y, Phuong HT, Iio K, Obika S, Fukushi M, Irie T, Tsuruta D, Sakamoto S, Tanaka K, Saeki Y, Fukai S, Tokunaga F
Communications biology 2020 Apr 3;3(1):163
Communications biology 2020 Apr 3;3(1):163
Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation.
Douglas T, Saleh M
The Journal of biological chemistry 2020 Apr 17;295(16):5216-5228
The Journal of biological chemistry 2020 Apr 17;295(16):5216-5228
The anticonvulsive Phenhydan(®) suppresses extrinsic cell death.
Moerke C, Jaco I, Dewitz C, Müller T, Jacobsen AV, Gautheron J, Fritsch J, Schmitz J, Bräsen JH, Günther C, Murphy JM, Kunzendorf U, Meier P, Krautwald S
Cell death and differentiation 2019 Sep;26(9):1631-1645
Cell death and differentiation 2019 Sep;26(9):1631-1645
A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.
Sun S, Li F, Liu Y, Qu H, Wong SW, Zeng L, Yu M, Feng H, Liu H, Han D
Archives of oral biology 2019 May;101:100-107
Archives of oral biology 2019 May;101:100-107
MST1 Negatively Regulates TNFα-Induced NF-κB Signaling through Modulating LUBAC Activity.
Lee IY, Lim JM, Cho H, Kim E, Kim Y, Oh HK, Yang WS, Roh KH, Park HW, Mo JS, Yoon JH, Song HK, Choi EJ
Molecular cell 2019 Mar 21;73(6):1138-1149.e6
Molecular cell 2019 Mar 21;73(6):1138-1149.e6
Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.
Douglas T, Saleh M
Cell reports 2019 Dec 10;29(11):3652-3663.e5
Cell reports 2019 Dec 10;29(11):3652-3663.e5
Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation.
Dondelinger Y, Delanghe T, Priem D, Wynosky-Dolfi MA, Sorobetea D, Rojas-Rivera D, Giansanti P, Roelandt R, Gropengiesser J, Ruckdeschel K, Savvides SN, Heck AJR, Vandenabeele P, Brodsky IE, Bertrand MJM
Nature communications 2019 Apr 15;10(1):1729
Nature communications 2019 Apr 15;10(1):1729
LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L.
Taraborrelli L, Peltzer N, Montinaro A, Kupka S, Rieser E, Hartwig T, Sarr A, Darding M, Draber P, Haas TL, Akarca A, Marafioti T, Pasparakis M, Bertin J, Gough PJ, Bouillet P, Strasser A, Leverkus M, Silke J, Walczak H
Nature communications 2018 Sep 25;9(1):3910
Nature communications 2018 Sep 25;9(1):3910
LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.
Peltzer N, Darding M, Montinaro A, Draber P, Draberova H, Kupka S, Rieser E, Fisher A, Hutchinson C, Taraborrelli L, Hartwig T, Lafont E, Haas TL, Shimizu Y, Böiers C, Sarr A, Rickard J, Alvarez-Diaz S, Ashworth MT, Beal A, Enver T, Bertin J, Kaiser W, Strasser A, Silke J, Bouillet P, Walczak H
Nature 2018 May;557(7703):112-117
Nature 2018 May;557(7703):112-117
Parkin regulates NF-κB by mediating site-specific ubiquitination of RIPK1.
Wang Y, Shan B, Liang Y, Wei H, Yuan J
Cell death & disease 2018 Jun 28;9(7):732
Cell death & disease 2018 Jun 28;9(7):732
OTULIN limits cell death and inflammation by deubiquitinating LUBAC.
Heger K, Wickliffe KE, Ndoja A, Zhang J, Murthy A, Dugger DL, Maltzman A, de Sousa E Melo F, Hung J, Zeng Y, Verschueren E, Kirkpatrick DS, Vucic D, Lee WP, Roose-Girma M, Newman RJ, Warming S, Hsiao YC, Kőműves LG, Webster JD, Newton K, Dixit VM
Nature 2018 Jul;559(7712):120-124
Nature 2018 Jul;559(7712):120-124
ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC.
Dziedzic SA, Su Z, Jean Barrett V, Najafov A, Mookhtiar AK, Amin P, Pan H, Sun L, Zhu H, Ma A, Abbott DW, Yuan J
Nature cell biology 2018 Jan;20(1):58-68
Nature cell biology 2018 Jan;20(1):58-68
Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2.
Annibaldi A, Wicky John S, Vanden Berghe T, Swatek KN, Ruan J, Liccardi G, Bianchi K, Elliott PR, Choi SM, Van Coillie S, Bertin J, Wu H, Komander D, Vandenabeele P, Silke J, Meier P
Molecular cell 2018 Feb 15;69(4):566-580.e5
Molecular cell 2018 Feb 15;69(4):566-580.e5
HOIL1 Is Essential for the Induction of Type I and III Interferons by MDA5 and Regulates Persistent Murine Norovirus Infection.
MacDuff DA, Baldridge MT, Qaqish AM, Nice TJ, Darbandi AD, Hartley VL, Peterson ST, Miner JJ, Iwai K, Virgin HW
Journal of virology 2018 Dec 1;92(23)
Journal of virology 2018 Dec 1;92(23)
Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice.
Peuhu E, Salomaa SI, De Franceschi N, Potter CS, Sundberg JP, Pouwels J
PloS one 2017;12(10):e0186628
PloS one 2017;12(10):e0186628
The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex.
Khan MH, Salomaa SI, Jacquemet G, Butt U, Miihkinen M, Deguchi T, Kremneva E, Lappalainen P, Humphries MJ, Pouwels J
Journal of cell science 2017 Sep 15;130(18):3094-3107
Journal of cell science 2017 Sep 15;130(18):3094-3107
A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.
Boege Y, Malehmir M, Healy ME, Bettermann K, Lorentzen A, Vucur M, Ahuja AK, Böhm F, Mertens JC, Shimizu Y, Frick L, Remouchamps C, Mutreja K, Kähne T, Sundaravinayagam D, Wolf MJ, Rehrauer H, Koppe C, Speicher T, Padrissa-Altés S, Maire R, Schattenberg JM, Jeong JS, Liu L, Zwirner S, Boger R, Hüser N, Davis RJ, Müllhaupt B, Moch H, Schulze-Bergkamen H, Clavien PA, Werner S, Borsig L, Luther SA, Jost PJ, Weinlich R, Unger K, Behrens A, Hillert L, Dillon C, Di Virgilio M, Wallach D, Dejardin E, Zender L, Naumann M, Walczak H, Green DR, Lopes M, Lavrik I, Luedde T, Heikenwalder M, Weber A
Cancer cell 2017 Sep 11;32(3):342-359.e10
Cancer cell 2017 Sep 11;32(3):342-359.e10
A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN.
Weber A, Elliott PR, Pinto-Fernandez A, Bonham S, Kessler BM, Komander D, El Oualid F, Krappmann D
Cell chemical biology 2017 Oct 19;24(10):1299-1313.e7
Cell chemical biology 2017 Oct 19;24(10):1299-1313.e7
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death.
Lafont E, Kantari-Mimoun C, Draber P, De Miguel D, Hartwig T, Reichert M, Kupka S, Shimizu Y, Taraborrelli L, Spit M, Sprick MR, Walczak H
The EMBO journal 2017 May 2;36(9):1147-1166
The EMBO journal 2017 May 2;36(9):1147-1166
Decreased linear ubiquitination of NEMO and FADD on apoptosis with caspase-mediated cleavage of HOIP.
Goto E, Tokunaga F
Biochemical and biophysical research communications 2017 Mar 25;485(1):152-159
Biochemical and biophysical research communications 2017 Mar 25;485(1):152-159
MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death.
Jaco I, Annibaldi A, Lalaoui N, Wilson R, Tenev T, Laurien L, Kim C, Jamal K, Wicky John S, Liccardi G, Chau D, Murphy JM, Brumatti G, Feltham R, Pasparakis M, Silke J, Meier P
Molecular cell 2017 Jun 1;66(5):698-710.e5
Molecular cell 2017 Jun 1;66(5):698-710.e5
SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitination.
Wei R, Xu LW, Liu J, Li Y, Zhang P, Shan B, Lu X, Qian L, Wu Z, Dong K, Zhu H, Pan L, Yuan J, Pan H
Genes & development 2017 Jun 1;31(11):1162-1176
Genes & development 2017 Jun 1;31(11):1162-1176
HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains.
Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nakano H, Tanaka Y, Iwai K, Inoue JI
PLoS pathogens 2017 Jan;13(1):e1006162
PLoS pathogens 2017 Jan;13(1):e1006162
SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland.
Peuhu E, Kaukonen R, Lerche M, Saari M, Guzmán C, Rantakari P, De Franceschi N, Wärri A, Georgiadou M, Jacquemet G, Mattila E, Virtakoivu R, Liu Y, Attieh Y, Silva KA, Betz T, Sundberg JP, Salmi M, Deugnier MA, Eliceiri KW, Ivaska J
The EMBO journal 2017 Jan 17;36(2):165-182
The EMBO journal 2017 Jan 17;36(2):165-182
Lack of interaction between NEMO and SHARPIN impairs linear ubiquitination and NF-κB activation and leads to incontinentia pigmenti.
Bal E, Laplantine E, Hamel Y, Dubosclard V, Boisson B, Pescatore A, Picard C, Hadj-Rabia S, Royer G, Steffann J, Bonnefont JP, Ursini VM, Vabres P, Munnich A, Casanova JL, Bodemer C, Weil R, Agou F, Smahi A
The Journal of allergy and clinical immunology 2017 Dec;140(6):1671-1682.e2
The Journal of allergy and clinical immunology 2017 Dec;140(6):1671-1682.e2
Roles of the TRAF6 and Pellino E3 ligases in MyD88 and RANKL signaling.
Strickson S, Emmerich CH, Goh ETH, Zhang J, Kelsall IR, Macartney T, Hastie CJ, Knebel A, Peggie M, Marchesi F, Arthur JSC, Cohen P
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):E3481-E3489
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):E3481-E3489
SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
Elliott PR, Leske D, Hrdinka M, Bagola K, Fiil BK, McLaughlin SH, Wagstaff J, Volkmar N, Christianson JC, Kessler BM, Freund SM, Komander D, Gyrd-Hansen M
Molecular cell 2016 Sep 15;63(6):990-1005
Molecular cell 2016 Sep 15;63(6):990-1005
SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death.
Schlicher L, Wissler M, Preiss F, Brauns-Schubert P, Jakob C, Dumit V, Borner C, Dengjel J, Maurer U
EMBO reports 2016 Oct;17(10):1485-1497
EMBO reports 2016 Oct;17(10):1485-1497
--LUBAC deficiency perturbs TLR3 signaling to cause immunodeficiency and autoinflammation.
Zinngrebe J, Rieser E, Taraborrelli L, Peltzer N, Hartwig T, Ren H, Kovács I, Endres C, Draber P, Darding M, von Karstedt S, Lemke J, Dome B, Bergmann M, Ferguson BJ, Walczak H
The Journal of experimental medicine 2016 Nov 14;213(12):2671-2689
The Journal of experimental medicine 2016 Nov 14;213(12):2671-2689
An Essential Role for SHARPIN in the Regulation of Caspase 1 Activity in Sepsis.
Nastase MV, Zeng-Brouwers J, Frey H, Hsieh LT, Poluzzi C, Beckmann J, Schroeder N, Pfeilschifter J, Lopez-Mosqueda J, Mersmann J, Ikeda F, Iozzo RV, Dikic I, Schaefer L
The American journal of pathology 2016 May;186(5):1206-20
The American journal of pathology 2016 May;186(5):1206-20
CYLD Limits Lys63- and Met1-Linked Ubiquitin at Receptor Complexes to Regulate Innate Immune Signaling.
Hrdinka M, Fiil BK, Zucca M, Leske D, Bagola K, Yabal M, Elliott PR, Damgaard RB, Komander D, Jost PJ, Gyrd-Hansen M
Cell reports 2016 Mar 29;14(12):2846-58
Cell reports 2016 Mar 29;14(12):2846-58
Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling.
Yang YK, Yang C, Chan W, Wang Z, Deibel KE, Pomerantz JL
The Journal of biological chemistry 2016 Dec 9;291(50):25921-25936
The Journal of biological chemistry 2016 Dec 9;291(50):25921-25936
SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
Kupka S, De Miguel D, Draber P, Martino L, Surinova S, Rittinger K, Walczak H
Cell reports 2016 Aug 30;16(9):2271-80
Cell reports 2016 Aug 30;16(9):2271-80
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Damgaard RB, Walker JA, Marco-Casanova P, Morgan NV, Titheradge HL, Elliott PR, McHale D, Maher ER, McKenzie ANJ, Komander D
Cell 2016 Aug 25;166(5):1215-1230.e20
Cell 2016 Aug 25;166(5):1215-1230.e20
Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis.
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F
Nature communications 2016 Aug 24;7:12547
Nature communications 2016 Aug 24;7:12547
The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice.
Douglas T, Champagne C, Morizot A, Lapointe JM, Saleh M
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2365-73
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2365-73
Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination.
de Almagro MC, Goncharov T, Newton K, Vucic D
Cell death & disease 2015 Jun 25;6(6):e1800
Cell death & disease 2015 Jun 25;6(6):e1800
Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia.
Boisson B, Laplantine E, Dobbs K, Cobat A, Tarantino N, Hazen M, Lidov HG, Hopkins G, Du L, Belkadi A, Chrabieh M, Itan Y, Picard C, Fournet JC, Eibel H, Tsitsikov E, Pai SY, Abel L, Al-Herz W, Casanova JL, Israel A, Notarangelo LD
The Journal of experimental medicine 2015 Jun 1;212(6):939-51
The Journal of experimental medicine 2015 Jun 1;212(6):939-51
LUBAC-Recruited CYLD and A20 Regulate Gene Activation and Cell Death by Exerting Opposing Effects on Linear Ubiquitin in Signaling Complexes.
Draber P, Kupka S, Reichert M, Draberova H, Lafont E, de Miguel D, Spilgies L, Surinova S, Taraborrelli L, Hartwig T, Rieser E, Martino L, Rittinger K, Walczak H
Cell reports 2015 Dec 15;13(10):2258-72
Cell reports 2015 Dec 15;13(10):2258-72
HOIP deficiency causes embryonic lethality by aberrant TNFR1-mediated endothelial cell death.
Peltzer N, Rieser E, Taraborrelli L, Draber P, Darding M, Pernaute B, Shimizu Y, Sarr A, Draberova H, Montinaro A, Martinez-Barbera JP, Silke J, Rodriguez TA, Walczak H
Cell reports 2014 Oct 9;9(1):153-165
Cell reports 2014 Oct 9;9(1):153-165
Binding of OTULIN to the PUB domain of HOIP controls NF-κB signaling.
Schaeffer V, Akutsu M, Olma MH, Gomes LC, Kawasaki M, Dikic I
Molecular cell 2014 May 8;54(3):349-61
Molecular cell 2014 May 8;54(3):349-61
Molecular basis and regulation of OTULIN-LUBAC interaction.
Elliott PR, Nielsen SV, Marco-Casanova P, Fiil BK, Keusekotten K, Mailand N, Freund SM, Gyrd-Hansen M, Komander D
Molecular cell 2014 May 8;54(3):335-48
Molecular cell 2014 May 8;54(3):335-48
TNFR1-dependent cell death drives inflammation in Sharpin-deficient mice.
Rickard JA, Anderton H, Etemadi N, Nachbur U, Darding M, Peltzer N, Lalaoui N, Lawlor KE, Vanyai H, Hall C, Bankovacki A, Gangoda L, Wong WW, Corbin J, Huang C, Mocarski ES, Murphy JM, Alexander WS, Voss AK, Vaux DL, Kaiser WJ, Walczak H, Silke J
eLife 2014 Dec 2;3
eLife 2014 Dec 2;3
Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.
Yang S, Wang B, Humphries F, Jackson R, Healy ME, Bergin R, Aviello G, Hall B, McNamara D, Darby T, Quinlan A, Shanahan F, Melgar S, Fallon PG, Moynagh PN
Nature immunology 2013 Sep;14(9):927-36
Nature immunology 2013 Sep;14(9):927-36
OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1-linked polyubiquitin.
Keusekotten K, Elliott PR, Glockner L, Fiil BK, Damgaard RB, Kulathu Y, Wauer T, Hospenthal MK, Gyrd-Hansen M, Krappmann D, Hofmann K, Komander D
Cell 2013 Jun 6;153(6):1312-26
Cell 2013 Jun 6;153(6):1312-26
OTULIN restricts Met1-linked ubiquitination to control innate immune signaling.
Fiil BK, Damgaard RB, Wagner SA, Keusekotten K, Fritsch M, Bekker-Jensen S, Mailand N, Choudhary C, Komander D, Gyrd-Hansen M
Molecular cell 2013 Jun 27;50(6):818-830
Molecular cell 2013 Jun 27;50(6):818-830
Disease-causing mutations in the XIAP BIR2 domain impair NOD2-dependent immune signalling.
Damgaard RB, Fiil BK, Speckmann C, Yabal M, zur Stadt U, Bekker-Jensen S, Jost PJ, Ehl S, Mailand N, Gyrd-Hansen M
EMBO molecular medicine 2013 Aug;5(8):1278-95
EMBO molecular medicine 2013 Aug;5(8):1278-95
The ubiquitin ligase XIAP recruits LUBAC for NOD2 signaling in inflammation and innate immunity.
Damgaard RB, Nachbur U, Yabal M, Wong WW, Fiil BK, Kastirr M, Rieser E, Rickard JA, Bankovacki A, Peschel C, Ruland J, Bekker-Jensen S, Mailand N, Kaufmann T, Strasser A, Walczak H, Silke J, Jost PJ, Gyrd-Hansen M
Molecular cell 2012 Jun 29;46(6):746-58
Molecular cell 2012 Jun 29;46(6):746-58
Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency.
Boisson B, Laplantine E, Prando C, Giliani S, Israelsson E, Xu Z, Abhyankar A, Israël L, Trevejo-Nunez G, Bogunovic D, Cepika AM, MacDuff D, Chrabieh M, Hubeau M, Bajolle F, Debré M, Mazzolari E, Vairo D, Agou F, Virgin HW, Bossuyt X, Rambaud C, Facchetti F, Bonnet D, Quartier P, Fournet JC, Pascual V, Chaussabel D, Notarangelo LD, Puel A, Israël A, Casanova JL, Picard C
Nature immunology 2012 Dec;13(12):1178-86
Nature immunology 2012 Dec;13(12):1178-86
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 14626-1-AP(SHARPIN antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SHARPIN antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SHARPIN. This antibody recognizes human,mouse,rat antigen. The SHARPIN antibody has been validated for the following applications: ELISA, WB, IP, IHC analysis.
