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- Product number
- 720290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRHR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references From the Cover: 2.45-GHz Microwave Radiation Impairs Hippocampal Learning and Spatial Memory: Involvement of Local Stress Mechanism-Induced Suppression of iGluR/ERK/CREB Signaling.
Shahin S, Banerjee S, Swarup V, Singh SP, Chaturvedi CM
Toxicological sciences : an official journal of the Society of Toxicology 2018 Feb 1;161(2):349-374
Toxicological sciences : an official journal of the Society of Toxicology 2018 Feb 1;161(2):349-374
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence was performed on HEK-293 cells transfected with CRHR1 expression plasmid. 24 hours post transection, cells were fixed and permeabilized for detection of CRHR1 using Anti-CRHR1 Rabbit Polyclonal Antibody (Product # 720290, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CRHR1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Corticotropin releasing hormone receptor 1. Panel e) shows no signal in untransfected cells. Panel f) represents transfected cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of endogenous Corticotropin releasing hormone receptor 1 / CRHR1 was performed on NIH/3T3 cells labeled with Anti-CRHR1 Rabbit Polyclonal Antibody (Product# 720290, 5 ug/ 1M cells) or with Rabbit isotype control and detected with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, (Alexa Fluor® 488 conjugate, Product# A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-Antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer (4468770).
