PA5-18801

antibody from Invitrogen Antibodies

Targeting: LINC02210-CRHR1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18801

Provider

Invitrogen Antibodies

Product name

CRHR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CRHR1 in Neuro2a cells using a CRHR1 polyclonal antibody (Product #PA5-18801). Neuro2a cells were permeabilized with 0.15% Triton. Cells were incubated with 10 µg/mL of primary antibody for one hour followed by an Alexa Fluor 488 secondary antibody at a concentration of 2 µg/mL. Plasma membrane and vesicle staining is shown in the image. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CRHR1 in MCF7 cells using a CRHR1 polyclonal antibody (Product #PA5-18801). MCF7 cells were permeabilized with 0.15% Triton. Cells were incubated with 10 µg/mL of primary antibody for one hour followed by an Alexa Fluor 488 secondary antibody at a concentration of 2 µg/mL. Cytoplasmic and vesicle staining can be seen as shown above. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL).