MA5-24016

antibody from Invitrogen Antibodies

Targeting: ECE1 ECE

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24016

Provider

Invitrogen Antibodies

Product name

ECE1 Monoclonal Antibody (303913)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute at 0.5 mg/mL in sterile PBS.

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

303913

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of MCF-7 human breast cancer cell line was stained with Rat Anti-human ECE-1 Monoclonal Antibody (Product # MA5-24016) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ECE1 in MCF-7 human breast cancer cell line. Samples were incubated in ECE1 monoclonal antibody (Product # MA5-24016) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.