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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-47201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human ECE-2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ECE1 in immersion fixed paraffin-embedded sections of human kidney. Samples were incubated in ECE1 polyclonal antibody (Product # PA5-47201) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells in glomeruli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 human breast cancer cell line was stained with human ECE-1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47201) or control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ECE1 in To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. Samples were incubated in ECE1 polyclonal antibody (Product # PA5-47201) or control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
