Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF1784 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ECE-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human ECE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human ECE-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P42892
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Retinal genes are differentially expressed in areas of primary versus secondary degeneration following partial optic nerve injury.
Endothelin-converting enzymes degrade α-synuclein and are reduced in dementia with Lewy bodies.
Endothelin-converting enzyme-1 (ECE-1) is post-transcriptionally regulated by alternative polyadenylation.
Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons.
Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain.
Endosomal endothelin-converting enzyme-1: a regulator of beta-arrestin-dependent ERK signaling.
Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins.
Chiha W, LeVaillant CJ, Bartlett CA, Hewitt AW, Melton PE, Fitzgerald M, Harvey AR
PloS one 2018;13(2):e0192348
PloS one 2018;13(2):e0192348
Endothelin-converting enzymes degrade α-synuclein and are reduced in dementia with Lewy bodies.
Miners JS, Love S
Journal of neurochemistry 2017 Apr;141(2):275-286
Journal of neurochemistry 2017 Apr;141(2):275-286
Endothelin-converting enzyme-1 (ECE-1) is post-transcriptionally regulated by alternative polyadenylation.
Whyteside AR, Turner AJ, Lambert DW
PloS one 2014;9(1):e83260
PloS one 2014;9(1):e83260
Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons.
Zhao P, Canals M, Murphy JE, Klingler D, Eriksson EM, Pelayo JC, Hardt M, Bunnett NW, Poole DP
The Journal of biological chemistry 2013 Sep 6;288(36):25689-700
The Journal of biological chemistry 2013 Sep 6;288(36):25689-700
Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain.
Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS
Journal of neurochemistry 2010 Oct;115(1):47-57
Journal of neurochemistry 2010 Oct;115(1):47-57
Endosomal endothelin-converting enzyme-1: a regulator of beta-arrestin-dependent ERK signaling.
Cottrell GS, Padilla BE, Amadesi S, Poole DP, Murphy JE, Hardt M, Roosterman D, Steinhoff M, Bunnett NW
The Journal of biological chemistry 2009 Aug 14;284(33):22411-25
The Journal of biological chemistry 2009 Aug 14;284(33):22411-25
Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins.
Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW
The Journal of cell biology 2007 Dec 3;179(5):981-97
The Journal of cell biology 2007 Dec 3;179(5):981-97
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ECE-1 in Human Kidney. ECE-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ECE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ECE-1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Human ECE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.