Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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- Product number
- GTX104577 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104577, RRID:AB_1950932
- Product name
- MMP2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells.
ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
Pin2/TRF1‑binding protein X1 inhibits colorectal cancer cell migration and invasion in vitro and metastasis in vivo via the nuclear factor‑κB signaling pathway.
Oxidative stress preconditioning of mouse perivascular myogenic progenitors selects a subpopulation of cells with a distinct survival advantage in vitro and in vivo.
Oxymatrine induces apoptosis and inhibits invasion in Gallbladder carcinoma via PTEN/PI3K/AKT pathway.
The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells.
Transforming growth factor alpha promotes tumorigenesis and regulates epithelial-mesenchymal transition modulation in colon cancer.
The invasiveness of human cervical cancer associated to the function of Na(V)1.6 channels is mediated by MMP-2 activity.
Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.
MicroRNA-194 modulates epithelial-mesenchymal transition in human colorectal cancer metastasis.
Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.
3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng.
Effects of Panax notoginseng on the Metastasis of Human Colorectal Cancer Cells.
The dual function of PRMT1 in modulating epithelial-mesenchymal transition and cellular senescence in breast cancer cells through regulation of ZEB1.
A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells.
Contrasting effects of stanniocalcin-related polypeptides on macrophage foam cell formation and vascular smooth muscle cell migration.
Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Monoacylglycerol lipase promotes metastases in nasopharyngeal carcinoma.
PRMT7 induces epithelial-to-mesenchymal transition and promotes metastasis in breast cancer.
Stimulatory effects of cardiotrophin 1 on atherosclerosis.
Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Huang C, Huang YL, Wang CC, Pan YL, Lai YH, Huang HC
Journal of agricultural and food chemistry 2019 Mar 13;67(10):2818-2830
Journal of agricultural and food chemistry 2019 Mar 13;67(10):2818-2830
ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
DaSilva-Arnold SC, Kuo CY, Davra V, Remache Y, Kim PCW, Fisher JP, Zamudio S, Al-Khan A, Birge RB, Illsley NP
Molecular human reproduction 2019 Feb 1;25(2):61-75
Molecular human reproduction 2019 Feb 1;25(2):61-75
Pin2/TRF1‑binding protein X1 inhibits colorectal cancer cell migration and invasion in vitro and metastasis in vivo via the nuclear factor‑κB signaling pathway.
Jiang T, Li H, Jiang R, Li H, Hou P, Chen Y, Bai J, Song J
Oncology reports 2018 Sep;40(3):1533-1544
Oncology reports 2018 Sep;40(3):1533-1544
Oxidative stress preconditioning of mouse perivascular myogenic progenitors selects a subpopulation of cells with a distinct survival advantage in vitro and in vivo.
Gargioli C, Turturici G, Barreca MM, Spinello W, Fuoco C, Testa S, Feo S, Cannata SM, Cossu G, Sconzo G, Geraci F
Cell death & disease 2018 Jan 3;9(1):1
Cell death & disease 2018 Jan 3;9(1):1
Oxymatrine induces apoptosis and inhibits invasion in Gallbladder carcinoma via PTEN/PI3K/AKT pathway.
Qian L, Li X, Ye P, Wang G, Dai W, Liu Y, Gao Q, Shen G
Cytotechnology 2018 Feb;70(1):83-94
Cytotechnology 2018 Feb;70(1):83-94
The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells.
Taglieri L, Rubinacci G, Giuffrida A, Carradori S, Scarpa S
Investigational new drugs 2018 Feb;36(1):28-35
Investigational new drugs 2018 Feb;36(1):28-35
Transforming growth factor alpha promotes tumorigenesis and regulates epithelial-mesenchymal transition modulation in colon cancer.
Yu CY, Chang WC, Zheng JH, Hung WH, Cho EC
Biochemical and biophysical research communications 2018 Dec 2;506(4):901-906
Biochemical and biophysical research communications 2018 Dec 2;506(4):901-906
The invasiveness of human cervical cancer associated to the function of Na(V)1.6 channels is mediated by MMP-2 activity.
Lopez-Charcas O, Espinosa AM, Alfaro A, Herrera-Carrillo Z, Ramirez-Cordero BE, Cortes-Reynosa P, Perez Salazar E, Berumen J, Gomora JC
Scientific reports 2018 Aug 29;8(1):12995
Scientific reports 2018 Aug 29;8(1):12995
Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.
DaSilva-Arnold SC, Zamudio S, Al-Khan A, Alvarez-Perez J, Mannion C, Koenig C, Luke D, Perez AM, Petroff M, Alvarez M, Illsley NP
Biology of reproduction 2018 Aug 1;99(2):409-421
Biology of reproduction 2018 Aug 1;99(2):409-421
MicroRNA-194 modulates epithelial-mesenchymal transition in human colorectal cancer metastasis.
Cai HK, Chen X, Tang YH, Deng YC
OncoTargets and therapy 2017;10:1269-1278
OncoTargets and therapy 2017;10:1269-1278
Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.
Wu PY, Lyu JL, Liu YJ, Chien TY, Hsu HC, Wen KC, Chiang HM
International journal of molecular sciences 2017 Oct 10;18(10)
International journal of molecular sciences 2017 Oct 10;18(10)
3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Chao W, Deng JS, Li PY, Liang YC, Huang GJ
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)
Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng.
Lee CY, Hsieh SL, Hsieh S, Tsai CC, Hsieh LC, Kuo YH, Wu CC
Oncology reports 2017 Jan;37(1):399-407
Oncology reports 2017 Jan;37(1):399-407
Effects of Panax notoginseng on the Metastasis of Human Colorectal Cancer Cells.
Hsieh SL, Hsieh S, Kuo YH, Wang JJ, Wang JC, Wu CC
The American journal of Chinese medicine 2016;44(4):851-70
The American journal of Chinese medicine 2016;44(4):851-70
The dual function of PRMT1 in modulating epithelial-mesenchymal transition and cellular senescence in breast cancer cells through regulation of ZEB1.
Gao Y, Zhao Y, Zhang J, Lu Y, Liu X, Geng P, Huang B, Zhang Y, Lu J
Scientific reports 2016 Jan 27;6:19874
Scientific reports 2016 Jan 27;6:19874
A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells.
Fu ZG, Wang L, Cui HY, Peng JL, Wang SJ, Geng JJ, Liu JD, Feng F, Song F, Li L, Zhu P, Jiang JL, Chen ZN
Oncotarget 2016 Feb 23;7(8):9429-47
Oncotarget 2016 Feb 23;7(8):9429-47
Contrasting effects of stanniocalcin-related polypeptides on macrophage foam cell formation and vascular smooth muscle cell migration.
Yamamoto K, Tajima Y, Hasegawa A, Takahashi Y, Kojima M, Watanabe R, Sato K, Shichiri M, Watanabe T
Peptides 2016 Aug;82:120-127
Peptides 2016 Aug;82:120-127
Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Huang CC, Wu DW, Lin PL, Lee H
Oncotarget 2015 Apr 20;6(11):8698-708
Oncotarget 2015 Apr 20;6(11):8698-708
Monoacylglycerol lipase promotes metastases in nasopharyngeal carcinoma.
Hu WR, Lian YF, Peng LX, Lei JJ, Deng CC, Xu M, Feng QS, Chen LZ, Bei JX, Zeng YX
International journal of clinical and experimental pathology 2014;7(7):3704-13
International journal of clinical and experimental pathology 2014;7(7):3704-13
PRMT7 induces epithelial-to-mesenchymal transition and promotes metastasis in breast cancer.
Yao R, Jiang H, Ma Y, Wang L, Wang L, Du J, Hou P, Gao Y, Zhao L, Wang G, Zhang Y, Liu DX, Huang B, Lu J
Cancer research 2014 Oct 1;74(19):5656-67
Cancer research 2014 Oct 1;74(19):5656-67
Stimulatory effects of cardiotrophin 1 on atherosclerosis.
Konii H, Sato K, Kikuchi S, Okiyama H, Watanabe R, Hasegawa A, Yamamoto K, Itoh F, Hirano T, Watanabe T
Hypertension (Dallas, Tex. : 1979) 2013 Nov;62(5):942-50
Hypertension (Dallas, Tex. : 1979) 2013 Nov;62(5):942-50
Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Hsu HY, Lin JH, Li CJ, Tsang SF, Tsai CH, Chyuan JH, Chiu SJ, Chuang SE
Evidence-based complementary and alternative medicine : eCAM 2012;2012:819632
Evidence-based complementary and alternative medicine : eCAM 2012;2012:819632
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX104577 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX104577 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MMP2 antibody detects MMP2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted by 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- U87-MG whole cell extract and conditioned medium (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GTX104577) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using MMP2(GTX104577) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of MMP2 protein from U87-MG whole cell extracts using 5 £gg of MMP2 antibody (GTX104577).Western blot analysis was performed using MMP2 antibody (GTX104577).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MMP2 antibody detects MMP2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MMP2 antibody (GTX104577) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MMP2 antibody detects MMP2 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MMP2 antibody (GTX104577) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MMP2 antibody detects MMP2 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MMP2 antibody (GTX104577) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MMP2 antibody detects MMP2 protein at extracellular matrix in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. MMP2 antibody (GTX104577) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MMP2 antibody detects MMP2 protein at extracellular matrix in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. MMP2 antibody (GTX104577) diluted at 1:500.