Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [11]
- Immunocytochemistry [2]
- Immunohistochemistry [10]
- Other assay [3]
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- Product number
- 10373-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- MCF7 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- WB result of MMP2 antibody (10373-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells.
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- MCF7 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- HepG2 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Rat heart tissue were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
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- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using (Product # 10373-2-AP) (MMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed MCF-7 cells using 10373-2-AP (MMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 40x lens).
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- Invitrogen Antibodies (provider)
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- Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:50 (under 40x lens).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 10x lens).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 10373-2-AP (MMP2 antibody) at dilution of 1:50 (under 40x lens).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10373-2-AP ( MMP2 Antibody) at dilution of 1:200 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10373-2-AP ( MMP2 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-MMP2 (IP:10373-2-AP, 4 ug; Detection:10373-2-AP 1:300) with MCF-7 cells lysate 2400 ug.
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- Invitrogen Antibodies (provider)
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- IP result of anti-MMP2 (IP:10373-2-AP, 3 ug; Detection:10373-2-AP 1:600) with NIH/3T3 cells lysate 2760 ug.
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- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-MMP2 (IP:10373-2-AP, 4ug; Detection:10373-2-AP 1:300) with NIH/3T3 cells lysate 1200ug.