Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 681802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681802, RRID:AB_2566497
- Product name
- Purified anti-MMP9
- Antibody type
- Monoclonal
- Antigen
- Human MMP-9, amino acids (Ala20-Asp707) (Accession# NP_004985.2), is expressed with a C-terminal 8His tag and a linker sequence in the 293E cell line.
- Description
- MMP9, or MMP-9, is a member of the matrix metalloprotease family of proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Proteolytic activities of MMPs play an important role in vascular remodeling, cellular migration, and the processing of ECM proteins and adhesion molecules. MMP9 consists of a prodomain, catalytic domain, hinge region, and a hemopexin domain. It is secreted as an inactive zymogen that becomes activated extracellularly. The most relevant natural activators of proMMP9 are unknown. MMP9 activation may be mediated by removal of the prodomain by serine proteases or other MMPs, or it may be a direct response to oxidative stress that disrupts the cysteine switch. MMP9 is capable of processing cytokines and chemokines. For example, it is reported to release the biologically active form of vascular endothelial growth factor (VEGF), which indicates that MMP9 may play a crucial role in the formation of new blood vessels. Increased MMP9 expression and activity has been shown to be related to various cancers, as it may contribute to the release of metastatic cells from the bulk tumor as well as their entrance at the site of metastasis by degrading proteins in the ECM and basement membrane.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- M2101G05
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Recombinant human MMP9 (lane 1, 50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP9 (clone M2101G05) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated