MA5-14228

antibody from Invitrogen Antibodies

Targeting: MMP9 CLG4B

Provider product page for MA5-14228

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [25]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]
Other assay [13]

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Product number: MA5-14228 - Provider product page
Provider: Invitrogen Antibodies
Product name: MMP9 Monoclonal Antibody (IIA5)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-14228 targets MMP-9 (92kDa Collagenase IV) in IHC, IP, and WB applications and shows reactivity with Human and Rat samples.
Antibody clone number: IIA5
Concentration: 0.2 mg/mL

MMP9 protein structure - MA5-14228 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3 Effect of KMUP-1 on LPS-induced inflammatory reaction proteins expression in RAW 264.7 cells. ( a ) Inducible nitric oxide synthase (iNOS), ( b ) TNF-alpha, ( c ) cyclooxygenase-2 (COX-2), ( d ) MMP-2, and MMP-9 were detected by Western blot analysis. Values are presented as mean +- SEM, n = 3. *** p < 0.001 compared to control group. # p < 0.05, ## p < 0.01 and ### p < 0.001 compared to LPS group.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 Distribution of Ki-67 ( a ) pRb- ( b ), MMP-9- ( c ) and Bax- ( d ) positive cells in the epithelium (Ep), loose ectomesenchyme condensations (lec), loose non-condensing ectomesenchyme (lnc) in formation of the secondary palate (7th-12th week). Data are shown as mean +- SD. Significant differences (Kruskal-Wallis) indicated by * p < 0.05, ** p < 0.001, *** p < 0.0001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 1 Cerebrovascular MMP9 expression in CAA-NH and CAA-ICH cases. Representative examples of MMP9 staining in a CAA-NH case ( a ), and a CAA-ICH case ( b ), representing the median values as shown in c. Leptomeningeal vessels are stained in both cases: in CAA-NH many vessels are only partially stained, whereas in CAA-ICH cases many vessels are fully stained. Overall, CAA-ICH cases had a significantly higher percentage of MMP9-stained leptomeningeal vessels (either fully or partially stained) compared to CAA-NH cases ( c ). The numbers of MMP9-stained cortical vessels did not differ between groups ( d ). Plots indicate median values with interquartile range. CAA-NH = CAA-non haemorrhagic, CAA-ICH = CAA-related ICH. *** p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 2 Quantification of MMP9-stained vessels in CAA-NH and CAA-ICH cases, classified according to staining grade. The percentage of leptomeningeal vessels with full ( a ), but not partial ( b ) MMP9 staining was higher in CAA-ICH cases compared to CAA-NH cases. Serial sections of a representative CAA-NH ( c , d ) and a representative CAA-ICH ( e , f ) case stained for Abeta ( c , e ) and MMP9 ( d , f ). Scale bar = 200 mum. Compared to CAA-NH cases, CAA-ICH cases had more fully Abeta-stained leptomeningeal vessels that were also fully stained for MMP9 ( g ), but fewer Abeta-stained vessels that had partial MMP9 staining ( h ). Plots indicate median values with interquartile range. CAA-NH = CAA-non haemorrhagic, CAA-ICH = CAA-related ICH. * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 5 The expression of MMP9 and TIMP3-stained vessels correlated with CAA grade. The percentage of fully MMP9-stained leptomeningeal ( a ) and number of fully MMP9-stained cortical ( b ) vessels showed a positive correlation with CAA grade. The percentage of fully TIMP3-stained leptomeningeal vessels did not significantly correlate to CAA grade ( c ), whereas the numbers of fully TIMP3-stained cortical ( d ) vessels showed a positive correlation with CAA grade. Solid circles = CAA-NH cases; open circles = CAA-ICH cases; r s = Spearman r

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 6 Disturbed balance of MMP9 and TIMP3 in vessels of CAA-ICH cases. The ratio of MMP9-stained (partially or fully) to TIMP3-stained (partially or fully) leptomeningeal vessels (a) and cortical vessels (b) was higher in CAA-ICH cases compared to CAA-NH casesIn CAA-NH cases, there was a positive correlation between the numbers of cortical vessels stained (partially or fully) for MMP9 and the numbers of cortical vessels stained (partially or fully) for TIMP3 ( c ). Such a correlation was not seen in CAA-ICH cases ( d ). Box plots show median values with the 25th and 75th percentile as boundaries and whiskers indicating minimum and maximum values. CAA-NH = CAA-non haemorrhagic, CAA-ICH = CAA-related ICH, * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 3 Photomicrograph of immunohistochemistry in the ileal sections for MMP-9, NF-kappaB and AQP-4. Anti-MMP-9 immunohistochemistry showing: (A) control group: minimal+ve immunoreaction (arrows) in some cells of the mucosal LP (B) 5-FU group: abundant+ve cytoplasmic immunoreaction (arrows) in the inflammatory cells within the mucosal C.T. and the epithelial cells covering the Vi. (C) Rh-treated group: minimal+ve immunoreaction (arrows) in few inflammatory and epithelial cells. Anti-NF-kappaB immunohistochemistry showing: (D) control group: few+ve cytoplasmic immunoreactions (arrows) in some cells of the mucosal C.T. (E) A 5-FU group: obvious increased+ve immunoreaction (arrows) involving both the cytoplasm and the nuclei of most of the epithelial and inflammatory cells (F) Rh-treated group: some epithelial and inflammatory cells showing+ve immunoreaction (arrows) which is mainly cytoplasmic with few nuclear immunoreactions. Anti-AQP-4 immunohistochemistry showing: (G) control group: obvious+ve AQP-4 immunoreaction (arrows) mostly in the basolateral regions in addition to the cytoplasm of the enterocytes. (H) A 5-FU group: minimal+ve AQP-4 immunoreaction (arrows) in the epithelial cells (I) Rh-treated group: increased+ve AQP-4 immunoreaction (arrows) mainly in the basolateral regions in addition to the cytoplasm of the enterocytes. Scale bars= 20 mum. AQP-4, aquaporin-4; C.T., connective tissue; LP, lamina propria; MMP-9, matrix metalloproteinase-9; NF-kappaB, nuclear factor-

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 TGF-beta-induced cell migration and invasion was reversed by knockdown of TAGLN . ( A ) Cell migration and invasion in HCT116 cells (left panel) and representative images of crystal violet staining (right panel). ( B ) Cell migration and invasion in HT29 cells (left panel) and representative images of crystal violet staining (right panel). ( C ) Representative images of Western blotting assay of E-cadherin, vimentin, fibronectin, MMP2, MMP9, and GADPH . 200 x magnification. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 Overexpression of HMGA2 restored altered CRC cell migration and invasion induced by inhibition of TAGLN . ( A ) Cell migration and invasion in HCT116 cells (left panel) and representative images of crystal violet staining (right panel). ( B ) Cell migration and invasion in HT29 cells (left panel) and representative images of crystal violet staining (right panel). ( C ) Representative images of Western blotting assay of nucleus and total TAGLN levels. ( D ) Representative images of Western blotting assay of E-cadherin, vimentin, fibronectin, MMP2, MMP9, and GADPH . 200 x magnification. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Effect of EMPA on MMP-2 and MMP-9 expression in the sciatic nerves in STZ-induced DPN in rats.Representative photomicrographs depicting ( a ) MMP-2 and ( b ) MMP-9 immunohistochemical staining in sciatic nerves (Scale bar is 50 mum). Panels represent the corresponding area % of ( c ) MMP-2 and ( d ) MMP-9 immunoexpression. Results are displayed as mean +- SD (n = 4). (*) vs CONT, (@) vs STZ, (#) vs EMPA; P < 0.05. CONT: control; DPN: diabetic peripheral neuropathy; DORS: dorsomorphin; EMPA: empagliflozin; MMP: metalloproteinase; STZ: streptozotocin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Micrograph illustrating the expression of c-Jun, acetylated c-Jun, and MMP-9 in representative PTC samples. The upper row of the micrograph represents cases with extra-thyroid invasion, showing: ( A ) weak widespread staining of c-Jun (low expression group), ( B ) strong diffuse staining of acetylated c-Jun in most tumor cells (high expression group), and ( C ) strong diffuse staining of MMP-9 in most tumor cells (high expression group). The lower row presents indolent cases of PTC, showing: ( D ) moderate staining of c-Jun in more than 40% of tumor cells (high expression group), ( E ) focal (up to 40% of tumor cells) staining of acetylated c-Jun (low expression group), and ( F ) weak widespread staining of MMP-9 (low expression group). Scale bars represent 50 um.