Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AB911 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-9 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human MMP-9 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12 and -13 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The TNF Family Molecules LIGHT and Lymphotoxin αβ Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2429-41
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2429-41
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Pégorier S, Wagner LA, Gleich GJ, Pretolani M
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 1;177(7):4861-9
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 1;177(7):4861-9
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Pégorier S, Wagner LA, Gleich GJ, Pretolani M
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 1;177(7):4861-9
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 1;177(7):4861-9
Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
Rouy D, Ernens I, Jeanty C, Wagner DR
Analytical biochemistry 2005 Mar 15;338(2):294-8
Analytical biochemistry 2005 Mar 15;338(2):294-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in MMP-9 transfected NS0 Mouse Cell Line. MMP-9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP-9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in Human Liver Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human MMP-9 Polyclonal Antibody (Catalog # AB911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.