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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA1-31526 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX22338-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX1 using a COX1 polyclonal antibody (Product # PA1-31526).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX1 was performed by loading 35 µg (in RIPA buffer) of mouse kidney (A) and skeletal muscle (B) lysates. Proteins were transferred to a membrane and probed with a COX1 Polyclonal Antibody (Product # PA1-31526) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX1 was performed by loading 35 µg (in RIPA buffer) of U937 lysates. Proteins were transferred to a membrane and probed with a COX1 Polyclonal Antibody (Product # PA1-31526) at a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX1 in PFA fixed NIH-3T3 cells. Cells were permeabilized with 0.15% Triton and COX1 Polyclonal Antibody (Product # PA1-31526) (Green) at a dilution of 10 µg/mL was used as a primary antibody. Blue: DAPI. Negative control : Unimmunized goat IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of COX1 in human heart tissue using COX1 Polyclonal Antibody (Product # PA1-31526) at a dilution of 3.8 µg/mL. Antigen retrieval : citrate buffer pH 6.
