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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-34813 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, Mouse brain, Rat brain. Predicted reactivity: Mouse (90%), Rat (88%), Dog (92%), Rabbit (93%), Sheep (93%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX1 using 50 µg mouse brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a COX1 polyclonal antibody (Product # PA5-34813) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX1 using 50 µg rat brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a COX1 polyclonal antibody (Product # PA5-34813) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX1 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a COX1 polyclonal antibody (Product # PA5-34813) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX1 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a COX1 Polyclonal Antibody (Product # PA5-34813) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COX1 Polyclonal Antibody detects PTGS1 protein at cytosol on by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. COX1 Polyclonal Antibody (Product # PA5-34813) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
